Abstract

) During the past funding period, a centralized Protein Purification and Expression core Facility (the PPE Core) has been a essential component of the Program. Operating under the day-to-day supervision of Dr. Dr. Hui Sun, the PPE Core Director, this core facility provided expertise and state-of-the-art equipment and material for protein purification and expression. The PPE core also maintains a central source of expression vectors and cell lines. The PPE Core has now been expanded to support three particular facets of research essential to the projects within this Program: 1. The PPE Core will continue to maintain state-of-the-art facilities for protein purification and expression; and to provide expertise in strategies and techniques of protein purification. It will also continue to maintain a collection of expression vectors, bacterial and yeast strains, resins for chromatography, and media for cell growth. 2. The PPE Core will provide a centralized point for submission of reactions for automated DNA sequence analysis by the W.H. Keck Center for Biotechnology Resources, Yale Medical School. 3. The PPE Core will support screens of high density matrix arrays for cell type-specific gene expression, providing shared access to this rapidly evolving technology.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
2P01CA016038-26
Application #
6101698
Study Section
Project Start
1999-05-07
Project End
2000-02-29
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
26
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
Yale University
Department
Type
DUNS #
082359691
City
New Haven
State
CT
Country
United States
Zip Code
06520

  Publications

Zhang, Pengwei; Monteiro da Silva, Gabriel; Deatherage, Catherine et al. (2018) Cell-Penetrating Peptide Mediates Intracellular Membrane Passage of Human Papillomavirus L2 Protein to Trigger Retrograde Trafficking. Cell 174:1465-1476.e13
Inoue, Takamasa; Zhang, Pengwei; Zhang, Wei et al. (2018) ?-Secretase promotes membrane insertion of the human papillomavirus L2 capsid protein during virus infection. J Cell Biol 217:3545-3559
Vallery, Tenaya K; Withers, Johanna B; Andoh, Joana A et al. (2018) Kaposi's Sarcoma-Associated Herpesvirus mRNA Accumulation in Nuclear Foci Is Influenced by Viral DNA Replication and Viral Noncoding Polyadenylated Nuclear RNA. J Virol 92:
Hayes, Karen E; Barr, Jamie A; Xie, Mingyi et al. (2018) Immunoprecipitation of Tri-methylated Capped RNA. Bio Protoc 8:
Park, Richard; Miller, George (2018) Epstein-Barr Virus-Induced Nodules on Viral Replication Compartments Contain RNA Processing Proteins and a Viral Long Noncoding RNA. J Virol 92:
Lipovsky, Alex; Erden, Asu; Kanaya, Eriko et al. (2017) The cellular endosomal protein stannin inhibits intracellular trafficking of human papillomavirus during virus entry. J Gen Virol 98:2821-2836
Singh, Gatikrushna; Fritz, Sarah M; Ranji, Arnaz et al. (2017) Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution. J Vis Exp :
Martinez, Ivan; Hayes, Karen E; Barr, Jamie A et al. (2017) An Exportin-1-dependent microRNA biogenesis pathway during human cell quiescence. Proc Natl Acad Sci U S A 114:E4961-E4970
Lee, Nara; Yario, Therese A; Gao, Jessica S et al. (2016) EBV noncoding RNA EBER2 interacts with host RNA-binding proteins to regulate viral gene expression. Proc Natl Acad Sci U S A 113:3221-6
DiMaio, Daniel (2016) Thank You, Edward. Merci, Louis. PLoS Pathog 12:e1005320

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