The long-term objective of the Scientific Core is to provide outstanding, state-of-the-art, cost-effective Core facilities and services that support all five projects of the Program. Core B is composed of a protein expression and purification facility, as well as other shared facilities including a darkroom, Phosphorlmager, ultracentrifuge, and liquid nitrogen freezers. The Core will also support the generation of retroviruses expressing variou~ shRNAs and microarray analysis of miRNA expression. The Phosphorlmager will be used for imaging and quantification of various results including primerextension assays, gel mobility shifts, and Westem blots. The protein expression and purification facility consists of a shaking incubator, a centrifuge, an FPLC, and protein expression vectors. It is used to express and purify proteins that are studied in each of the projects. In a new service, the Core will also generate shRNA constructs for the various projects. This will include design of shRNAs that target specific genes, cloning the inserts into retrovirus vectors, and generation of high-titer retrovirus stocks. The Core will also support the use of a Taqman microRNA microarray set-up by purchasing cassettes for the analysis. An Amaxa Nucleofector transfection apparatus will also be purchased for use by the projects. The Core also possesses liquid nitrogen freezers for the storage of cell lines and a gel documentation system. In addition, expertise is provided to all member laboratories by personnel who oversee the Core. This Scientific Core is essential to the success of the Program, and facilitates interaction between member laboratories.

Public Health Relevance

This interdisciplinary Program consists of five projects using diverse, state-of-the-art approaches. However, several projects use the same technologies, which are most efficiently provided in a cost-effective manner by staff with specialized expertise. This Core will provide these scientific services to all of the projects.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA016038-40
Application #
8476997
Study Section
Special Emphasis Panel (ZCA1-GRB-S)
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
40
Fiscal Year
2013
Total Cost
$62,682
Indirect Cost
$24,384
Name
Yale University
Department
Type
DUNS #
043207562
City
New Haven
State
CT
Country
United States
Zip Code
06520
Luo, Yong; Motamedi, Nasim; Magaldi, Thomas G et al. (2016) Interaction between Simian Virus 40 Major Capsid Protein VP1 and Cell Surface Ganglioside GM1 Triggers Vacuole Formation. MBio 7:e00297
Gorres, Kelly L; Daigle, Derek; Mohanram, Sudharshan et al. (2016) Valpromide Inhibits Lytic Cycle Reactivation of Epstein-Barr Virus. MBio 7:e00113
Brown, Jessica A; Kinzig, Charles G; DeGregorio, Suzanne J et al. (2016) Hoogsteen-position pyrimidines promote the stability and function of the MALAT1 RNA triple helix. RNA 22:743-9
Zhang, Wei; Xie, Mingyi; Shu, Mei-Di et al. (2016) A proximity-dependent assay for specific RNA-protein interactions in intact cells. RNA 22:1785-1792
Pawlica, Paulina; Moss, Walter N; Steitz, Joan A (2016) Host miRNA degradation by Herpesvirus saimiri small nuclear RNA requires an unstructured interacting region. RNA 22:1181-9
DiMaio, Daniel (2016) Thank You, Edward. Merci, Louis. PLoS Pathog 12:e1005320
Lee, Nara; Yario, Therese A; Gao, Jessica S et al. (2016) EBV noncoding RNA EBER2 interacts with host RNA-binding proteins to regulate viral gene expression. Proc Natl Acad Sci U S A 113:3221-6
Tycowski, Kazimierz T; Shu, Mei-Di; Steitz, Joan A (2016) Myriad Triple-Helix-Forming Structures in the Transposable Element RNAs of Plants and Fungi. Cell Rep 15:1266-76
Brown, Jessica A; Kinzig, Charles G; DeGregorio, Suzanne J et al. (2016) Methyltransferase-like protein 16 binds the 3'-terminal triple helix of MALAT1 long noncoding RNA. Proc Natl Acad Sci U S A 113:14013-14018
Guo, Yang Eric; Oei, Theresa; Steitz, Joan A (2015) Herpesvirus saimiri MicroRNAs Preferentially Target Host Cell Cycle Regulators. J Virol 89:10901-11

Showing the most recent 10 out of 331 publications