Core 2 is essential for the delivery of optimal veterinary care and facilities utilized for the conduct of in vivo experimentation. The Pl.'s of this program project will have access to the Division of Comparative Medicine's AAALAC accredited, centrally managed and operated 175,000 sq. ft. of space, located in five buildings at the Institute. Each of the units has been built or extensively renovated in the last eight years. Four of these buildings have three corridor systems and three have specialized bio-containment units for infectious or hazardous chemical experimentation. Barrier facilities for housing specific pathogen free transgenic mouse colonies are also available. These animal facilities provide MIT investigators a controlled environment with the necessary space, equipment, and containment facilities for performing biomedical related research in animals including germ-free work and also ensures personnel safety during the experimental process. The DCM Comparative Pathology Laboratory will also provide the personnel and expertise to perform histological and immunocytochemical analysis of animal tissues delivered from in vivo studies performed within the framework of the program project. Specifically the role of Core 2 is to provide facilities, equipment, and personnel with expertise to centrally manage and supervise animal husbandry and experimental animal manipulations and provide histopathologic and transgenic services involved in studies conducted in the Program Project Grant.
The specific aims are as follows:
Specific Aim #1 To provide histology, immunohistochemistry, pathology and molecular characterization expertise for extensive phenotyping and assessment of pathological damage of mouse models used in this program Specific Aim #2 To rederive transgenic and other specialized mouse strains by embryo transfer to assure specific pathogen free status of GEM used in this program Specific Aim #3 Provide colony management of mouse models for use by program project PI's and as needed generate transgenic animals by pronuclear microinjection of DMA constructs, embryonic stem cell manipulation, or gene silencing using RNAi technology.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA026731-34
Application #
8567218
Study Section
Special Emphasis Panel (ZCA1-GRB-P)
Project Start
Project End
2014-12-31
Budget Start
2013-01-01
Budget End
2013-12-31
Support Year
34
Fiscal Year
2013
Total Cost
$192,365
Indirect Cost
$77,434
Name
Massachusetts Institute of Technology
Department
Type
DUNS #
001425594
City
Cambridge
State
MA
Country
United States
Zip Code
02139
Whary, Mark T; Muthupalani, Sureshkumar; Ge, Zhongming et al. (2014) Helminth co-infection in Helicobacter pylori infected INS-GAS mice attenuates gastric premalignant lesions of epithelial dysplasia and glandular atrophy and preserves colonization resistance of the stomach to lower bowel microbiota. Microbes Infect 16:345-55
Kiraly, Orsolya; Gong, Guanyu; Roytman, Megan D et al. (2014) DNA glycosylase activity and cell proliferation are key factors in modulating homologous recombination in vivo. Carcinogenesis 35:2495-502
Lertpiriyapong, Kvin; Handt, Laurence; Feng, Yan et al. (2014) Pathogenic properties of enterohepatic Helicobacter spp. isolated from rhesus macaques with intestinal adenocarcinoma. J Med Microbiol 63:1004-16
Ge, Zhongming; Feng, Yan; Muthupalani, Sureshkumar et al. (2014) Helicobacter hepaticus cholesterol-?-glucosyltransferase is essential for establishing colonization in male A/JCr mice. Helicobacter 19:280-8
Shrivastav, Nidhi; Fedeles, Bogdan I; Li, Deyu et al. (2014) A chemical genetics analysis of the roles of bypass polymerase DinB and DNA repair protein AlkB in processing N2-alkylguanine lesions in vivo. PLoS One 9:e94716
Fox, James G; Wang, Timothy C (2014) Dietary factors modulate Helicobacter-associated gastric cancer in rodent models. Toxicol Pathol 42:162-81
Kumar, Yadunanda; Liang, Cui; Limmon, Gino V et al. (2014) Molecular analysis of serum and bronchoalveolar lavage in a mouse model of influenza reveals markers of disease severity that can be clinically useful in humans. PLoS One 9:e86912
Singh, Vipender; Peng, Chunte Sam; Li, Deyu et al. (2014) Direct observation of multiple tautomers of oxythiamine and their recognition by the thiamine pyrophosphate riboswitch. ACS Chem Biol 9:227-36
Li, Deyu; Fedeles, Bogdan I; Singh, Vipender et al. (2014) Tautomerism provides a molecular explanation for the mutagenic properties of the anti-HIV nucleoside 5-aza-5,6-dihydro-2'-deoxycytidine. Proc Natl Acad Sci U S A 111:E3252-9
Swennes, Alton G; Sheh, Alexander; Parry, Nicola M A et al. (2014) Helicobacter hepaticus infection promotes hepatitis and preneoplastic foci in farnesoid X receptor (FXR) deficient mice. PLoS One 9:e106764

Showing the most recent 10 out of 305 publications