The goals of Core A are to provide cellular and molecular assays in support of all projects. The technologies and methods employed include flow and image cytometry;genetic analyses, cell and tissue culture support and development of common reagents. This Core and its personnel have had a long and productive history of application of specialized cell and molecular assays to the study of Werner Syndrome. These notably include flow and image cytometric assays of cell cycle, survival, DNA damage and telomere status, reagents and assays for Immunologic probes and gene silencing, as well as cell culture support for the broad variety of in vitro assays that is encompassed by this work. Both the characterization of WRN RecQ protein activities and the consequences of these activities on cellular phenotypes thus rely on the use of cell and molecular assays that are used in com man by all of the Projects within this P01 renewal application. The implementation, enhancement and where needed, development of these assays is a service will be most effectively and efficiently performed by this specialized Core in order to optimize their use by the P01 Projects.

Public Health Relevance

Both the characterization of WRN RecQ protein activities and the consequences of these activities on cellular phenotypes rely on the use of Core A cell and molecular assays that are used in common by all of the Projects within this Program Project.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Research Program Projects (P01)
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Special Emphasis Panel (ZCA1-GRB-S)
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University of Washington
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Shiovitz, Stacey; Bertagnolli, Monica M; Renfro, Lindsay A et al. (2014) CpG island methylator phenotype is associated with response to adjuvant irinotecan-based therapy for stage III colon cancer. Gastroenterology 147:637-45
Shen, Jiang-Cheng; Fox, Edward J; Ahn, Eun Hyun et al. (2014) A rapid assay for measuring nucleotide excision repair by oligonucleotide retrieval. Sci Rep 4:4894
Yu, M; Trobridge, P; Wang, Y et al. (2014) Inactivation of TGF-* signaling and loss of PTEN cooperate to induce colon cancer in vivo. Oncogene 33:1538-47
Luo, Yanxin; Wong, Chao-Jen; Kaz, Andrew M et al. (2014) Differences in DNA methylation signatures reveal multiple pathways of progression from adenoma to colorectal cancer. Gastroenterology 147:418-29.e8
Kamath-Loeb, Ashwini S; Balakrishna, Sharath; Whittington, Dale et al. (2014) Sphingosine, a modulator of human translesion DNA polymerase activity. J Biol Chem 289:21663-72
Lauper, Julia M; Monnat Jr, Raymond J (2014) Diabetes mellitus and cancer in Werner syndrome. Acta Diabetol 51:159-61
Pavelitz, Thomas; Renfro, Lindsay; Foster, Nathan R et al. (2014) MRE11-deficiency associated with improved long-term disease free survival and overall survival in a subset of stage III colon cancer patients in randomized CALGB 89803 trial. PLoS One 9:e108483
Kiianitsa, Kostantin; Maizels, Nancy (2014) Ultrasensitive isolation, identification and quantification of DNA-protein adducts by ELISA-based RADAR assay. Nucleic Acids Res 42:e108
Gray, Lucas T; Vallur, Aarthy C; Eddy, Johanna et al. (2014) G quadruplexes are genomewide targets of transcriptional helicases XPB and XPD. Nat Chem Biol 10:313-8
Grady, William M; Pritchard, Colin C (2014) Molecular alterations and biomarkers in colorectal cancer. Toxicol Pathol 42:124-39

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