The Cellular, Molecular and Morphological Core provides a wide range of essential services for the Program Project. We have assembled, as part of our program, a team of supporting cellular and molecular biologists to assist us in the investigation of the molecular aspects of solid tumor physiology. The use of the services of Core B for 81 of 90 original articles published since 2006 provides the strongest evidence of the benefit of the Core to the Program. The molecular biology component will provide the expertise, apparatus, assay protocols, and essential reagents for the characterization of gene expression and regulation. These routine services include isolation and purification of DNA, RNA and proteins, in situ hybridization. Southern, Northern and Western blot analysis, the generation of DNA constructs, promoter analysis, the modification of gene expression in tumor and host stromal cell lines, and genotyping of transgenic animals. The cell culture component will provide the expertise and services, including the maintenance of parental and transfected cell lines, quality control, and maintenance of supplies. The morphology component will provide equipment, supplies and protocols as well as expertise and services for the analysis of tissues and cells, including fixation, embedding and sections preparation, as well as performing routine staining and immunohistochemical procedures. The flow cytometry component will provide equipment, supplies, protocol and services for characterization of specific cell populations. In addition to providing these services, the Core personnel will interact with Project Investigators to provide strategic consultation in the design of experimental procedures, to introduce innovative approaches using state-of-the-art techniques, and to assist in the completion of experiments and interpretation of data for molecular, cell and morphology studies. Finally, the Core personnel will assure reagent and antibody quality, manage biological information, acquire and store research materials, and maintain logs.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA080124-13
Application #
8657996
Study Section
Special Emphasis Panel (ZCA1-RPRB-O)
Project Start
Project End
Budget Start
2014-05-01
Budget End
2015-04-30
Support Year
13
Fiscal Year
2014
Total Cost
$364,751
Indirect Cost
$155,124
Name
Massachusetts General Hospital
Department
Type
DUNS #
073130411
City
Boston
State
MA
Country
United States
Zip Code
02199
Padera, Timothy P; Meijer, Eelco F J; Munn, Lance L (2016) The Lymphatic System in Disease Processes and Cancer Progression. Annu Rev Biomed Eng 18:125-58
Datta, Meenal; Via, Laura E; Chen, Wei et al. (2016) Mathematical Model of Oxygen Transport in Tuberculosis Granulomas. Ann Biomed Eng 44:863-72
Kloepper, Jonas; Riedemann, Lars; Amoozgar, Zohreh et al. (2016) Ang-2/VEGF bispecific antibody reprograms macrophages and resident microglia to anti-tumor phenotype and prolongs glioblastoma survival. Proc Natl Acad Sci U S A 113:4476-81
Chng, Kern Rei; Chan, Sock Hoai; Ng, Amanda Hui Qi et al. (2016) Tissue Microbiome Profiling Identifies an Enrichment of Specific Enteric Bacteria in Opisthorchis viverrini Associated Cholangiocarcinoma. EBioMedicine 8:195-202
Incio, Joao; Liu, Hao; Suboj, Priya et al. (2016) Obesity-Induced Inflammation and Desmoplasia Promote Pancreatic Cancer Progression and Resistance to Chemotherapy. Cancer Discov 6:852-69
Kunert, Christian; Baish, James W; Liao, Shan et al. (2016) Reply to Davis: Nitric oxide regulates lymphatic contractions. Proc Natl Acad Sci U S A 113:E106
Park, Kyung Ran; Monsky, Wayne L; Lee, Chang Geol et al. (2016) Mast Cells Contribute to Radiation-Induced Vascular Hyperpermeability. Radiat Res 185:182-9
Singhal, Prabhat K; Sassi, Slim; Lan, Lan et al. (2016) Mouse embryonic fibroblasts exhibit extensive developmental and phenotypic diversity. Proc Natl Acad Sci U S A 113:122-7
Askoxylakis, Vasileios; Ferraro, Gino B; Kodack, David P et al. (2016) Preclinical Efficacy of Ado-trastuzumab Emtansine in the Brain Microenvironment. J Natl Cancer Inst 108:
Pinter, Matthias; Trauner, Michael; Peck-Radosavljevic, Markus et al. (2016) Cancer and liver cirrhosis: implications on prognosis and management. ESMO Open 1:e000042

Showing the most recent 10 out of 248 publications