The overall goal of the Tissue Analysis Laboratory ("the Core") is to provide technical and professional support for each Project. The Core at the University of Virginia has been in place for the past decade of the two previously funded prostate POI programs. All assessments will be made by two highly skilled, experienced genitourinary pathologists with expertise in examining mouse and human prostate tissues and in immunohistochemistry. Dr. Frierson will provide overall direction ofthe Core laboratory at the University of Virginia, while Dr. Lucia will lead the laboratory at the University of Colorado. The Core will have a large impact on the POI. as it provides tissue examination for in vivo models that compliment in vitro experiments. Comparative analysis of murine prostate cancers and clinical human prostate cancer specimens from different patient cohorts from two different laboratories will impact the field by clarifying signaling pathways in prostate cancer progression. The Core will have 3 Aims:
Aim 1. Histologic analysis of cell cultures, xenografts, mouse models, and clinical human prostate tissue specimens. Specimens will be processed as archival (formalin-fixed, paraffin-embedded) samples or as frozen sections. In addition, Drs. Frierson and Lucia will use their expertise as pathologists to examine the prostate glands of transgenic mice to determine histopathologic changes induced by the genetic modifications.
Aim 2. Immunohistochemical analysis and optimization of antibodies, specifically to determine their utility in formalin-fixed, paraffin-embedded material and. if necessary, in frozen sections. Drs. Frierson and Lucia will use the avidin-biotin immunoperoxidase technique and their immunohistochemical expertise to determine optimal reagent conditions and antibody staining in cell cultures, xenografts, mouse models, and clinical human prostate cancer specimens.
Aim 3. Selection, preparation, and procurement of cells for laser microdissection. Drs. Frierson and Lucia have the ability to perform laser microdissection of prostate glands from human prostate tissues, transgenic mice, and xenografts for molecular studies

Public Health Relevance

Core C will function as a tissue analysis laboratory to examine specimens from mouse models of prostate cancer. In addition, the laboratory will undertake translational studies in which human prostate cancers will be examined for important molecules which have been uncovered in in vitro and animal models of aggressive prostate cancer. Such analysis will lead to a better understanding ofthe molecular underpinnings of human prostate cancer.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Research Program Projects (P01)
Project #
Application #
Study Section
Special Emphasis Panel (ZCA1-RPRB-O (J1))
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Virginia
United States
Zip Code
Dey, Bijan K; Pfeifer, Karl; Dutta, Anindya (2014) The H19 long noncoding RNA gives rise to microRNAs miR-675-3p and miR-675-5p to promote skeletal muscle differentiation and regeneration. Genes Dev 28:491-501
Yan, Chao; Liu, Degang; Li, Liwei et al. (2014) Discovery and characterization of small molecules that target the GTPase Ral. Nature 515:443-7
Zhang, Y; Kim, J; Mueller, A C et al. (2014) Multiple receptor tyrosine kinases converge on microRNA-134 to control KRAS, STAT5B, and glioblastoma. Cell Death Differ 21:720-34
Negishi, Masamitsu; Wongpalee, Somsakul P; Sarkar, Sukumar et al. (2014) A new lncRNA, APTR, associates with and represses the CDKN1A/p21 promoter by recruiting polycomb proteins. PLoS One 9:e95216
Kumar, Pankaj; Anaya, Jordan; Mudunuri, Suresh B et al. (2014) Meta-analysis of tRNA derived RNA fragments reveals that they are evolutionarily conserved and associate with AGO proteins to recognize specific RNA targets. BMC Biol 12:78
Bettegowda, Chetan; Sausen, Mark; Leary, Rebecca J et al. (2014) Detection of circulating tumor DNA in early- and late-stage human malignancies. Sci Transl Med 6:224ra24
Dancik, Garrett M; Owens, Charles R; Iczkowski, Kenneth A et al. (2014) A cell of origin gene signature indicates human bladder cancer has distinct cellular progenitors. Stem Cells 32:974-82
Nickerson, Michael L; Dancik, Garrett M; Im, Kate M et al. (2014) Concurrent alterations in TERT, KDM6A, and the BRCA pathway in bladder cancer. Clin Cancer Res 20:4935-48
Floyd, Desiree Hunt; Zhang, Ying; Dey, Bijan K et al. (2014) Novel anti-apoptotic microRNAs 582-5p and 363 promote human glioblastoma stem cell survival via direct inhibition of caspase 3, caspase 9, and Bim. PLoS One 9:e96239
Sun, D; Layer, R; Mueller, A C et al. (2014) Regulation of several androgen-induced genes through the repression of the miR-99a/let-7c/miR-125b-2 miRNA cluster in prostate cancer cells. Oncogene 33:1448-57

Showing the most recent 10 out of 42 publications