The Protein Biochemistry Core will provide state-of-the-art technical support in protein expression and purification, protein-protein interaction studies, immunoprecipitation, and enzymatic assays germane for the research goals being pursued in individual projects. By centralizing these services under the leadership of two experienced investigators, Patrick Sung (Core Director) and Steven Raynard (Core Co-ordinator), the Protein Biochemistry Core will enhance the productivity of program project members and promote synergy among the different projects by streamlining access of critical reagents and methodologies across laboratories. Following established protocols, the Protein Biochemistry Core personnel will carry out protein expression in bacteria, yeast, or insect cells and protein purification. Moreover, the Core will work with the participating laboratories to develop new protein expression and purification procedures. To help meet new challenges of the program, the Core Director and Core Co-ordinator will consult with program members on matters pertaining to protein-protein interaction studies, immunoprecipitation, and the development of enzyme assays.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Research Program Projects (P01)
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Special Emphasis Panel (ZCA1-GRB-S)
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Yale University
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Penketh, Philip G; Shyam, Krishnamurthy; Baumann, Raymond P et al. (2016) When alcohol is the answer: Trapping, identifying and quantifying simple alkylating species in aqueous environments. Anal Biochem 508:34-7
Penketh, P G; Shyam, K; Baumann, R P et al. (2015) A simple and inexpensive method to control oxygen concentrations within physiological and neoplastic ranges. Anal Biochem 491:1-3
Lin, Z Ping; Ratner, Elena S; Whicker, Margaret E et al. (2014) Triapine disrupts CtIP-mediated homologous recombination repair and sensitizes ovarian cancer cells to PARP and topoisomerase inhibitors. Mol Cancer Res 12:381-393
Penketh, Philip G; Shyam, Krishnamurthy; Zhu, Rui et al. (2014) Influence of phosphate and phosphoesters on the decomposition pathway of 1,2-bis(methylsulfonyl)-1-(2-chloroethyhydrazine (90CE), the active anticancer moiety generated by Laromustine, KS119, and KS119W. Chem Res Toxicol 27:818-33
Lamb, Kristy L; Liu, Yanfeng; Ishiguro, Kimiko et al. (2014) Tumor-associated mutations in O? -methylguanine DNA-methyltransferase (MGMT) reduce DNA repair functionality. Mol Carcinog 53:201-10
Penketh, Philip G; Patridge, Eric; Shyam, Krishnamurthy et al. (2014) Influence of glutathione and glutathione S-transferases on DNA interstrand cross-link formation by 1,2-bis(methylsulfonyl)-1-(2-chloroethyl)hydrazine, the active anticancer moiety generated by laromustine. Chem Res Toxicol 27:1440-9
Zhu, Rui; Baumann, Raymond P; Penketh, Philip G et al. (2013) Hypoxia-selective O6-alkylguanine-DNA alkyltransferase inhibitors: design, synthesis, and evaluation of 6-(benzyloxy)-2-(aryldiazenyl)-9H-purines as prodrugs of O6-benzylguanine. J Med Chem 56:1355-9
Zhu, Rui; Baumann, Raymond P; Patridge, Eric et al. (2013) Chloroethylating and methylating dual function antineoplastic agents display superior cytotoxicity against repair proficient tumor cells. Bioorg Med Chem Lett 23:1853-9
Daley, James M; Niu, Hengyao; Sung, Patrick (2013) Roles of DNA helicases in the mediation and regulation of homologous recombination. Adv Exp Med Biol 767:185-202
Daley, James M; Sung, Patrick (2013) RIF1 in DNA break repair pathway choice. Mol Cell 49:840-1

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