The signaling cascades of the Eph receptor tyrosine kinase family and the ephrin ligands have been implicated in pathological forms of angiogenesis and tumorigenesis. While initial investigations have focused on the role of this protein-protein interaction in axonal guidance, cell attachment and motility, there is now significant evidence for the overexpression and dysregulation of this interaction in numerous cancerous tissues. The key research hypothesis of this proposal is that a limited set of structural determinants are responsible for the promiscuity and selectivity of ligand recognition exhibited by the Eph receptor family, and that these structural determinants can be exploited for small molecule modulators of this interaction. The proposed research will result in a demonstrated description and characterization of the mode of binding across this large family of receptor-ligand interactions. The outcome will be translated in a discovery approach to the development of small molecule compounds that exploit these structural determinants.
Aim 1 : Evaluate structural determinants of specificity versus promiscuity in Eph receptor-ligand binding by using X-ray crystallography.
Aim 2 : Develop strategies to stabilize the flexible Eph receptor J-K loop in the ephrin-binding cleft to facilitate receptor crystallization in complex with small molecular weight chemical compounds.
Aim 3 : Characterize and improve small molecular weight chemical compounds identified in high throughput screens and virtual ligand screens for inhibitors of EphB2 and EphB4 receptor ligand binding.
The Eph receptor tyrosine kinases and their ephrin ligands are involved in tumor growth and represent an important asset of cancer targets. Defining the structural features responsible for Eph receptor ligand specificity and affinity will enable the development of new peptide and small molecules as potenfial cancer. therapeufic starting points.
|Hassan-Mohamed, I; Giorgio, C; Incerti, M et al. (2014) UniPR129 is a competitive small molecule Eph-ephrin antagonist blocking in vitro angiogenesis at low micromolar concentrations. Br J Pharmacol 171:5195-208|
|Vargas, Lina M; Leal, Nancy; Estrada, Lisbell D et al. (2014) EphA4 activation of c-Abl mediates synaptic loss and LTP blockade caused by amyloid-? oligomers. PLoS One 9:e92309|
|Lamberto, Ilaria; Lechtenberg, Bernhard C; Olson, Erika J et al. (2014) Development and structural analysis of a nanomolar cyclic peptide antagonist for the EphA4 receptor. ACS Chem Biol 9:2787-95|
|Barile, Elisa; Wang, Si; Das, Swadesh K et al. (2014) Design, synthesis and bioevaluation of an EphA2 receptor-based targeted delivery system. ChemMedChem 9:1403-12|
|Incerti, Matteo; Tognolini, Massimiliano; Russo, Simonetta et al. (2013) Amino acid conjugates of lithocholic acid as antagonists of the EphA2 receptor. J Med Chem 56:2936-47|
|Wang, Si; Noberini, Roberta; Stebbins, John L et al. (2013) Targeted delivery of paclitaxel to EphA2-expressing cancer cells. Clin Cancer Res 19:128-37|
|Mercurio, Flavia A; Marasco, Daniela; Pirone, Luciano et al. (2013) Heterotypic Sam-Sam association between Odin-Sam1 and Arap3-Sam: binding affinity and structural insights. Chembiochem 14:100-6|
|Duggineni, Srinivas; Mitra, Sayantan; Noberini, Roberta et al. (2013) Design, synthesis and characterization of novel small molecular inhibitors of ephrin-B2 binding to EphB4. Biochem Pharmacol 85:507-13|
|Wu, Bainan; Zhang, Ziming; Noberini, Roberta et al. (2013) HTS by NMR of combinatorial libraries: a fragment-based approach to ligand discovery. Chem Biol 20:19-33|
|Duggineni, Srinivas; Mitra, Sayantan; Lamberto, Ilaria et al. (2013) Design and Synthesis of Potent Bivalent Peptide Agonists Targeting the EphA2 Receptor. ACS Med Chem Lett 4:|
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