Abstract

The purpose of the Recombinant Virus Core C is to generate quality controlled recombinant herpesviruses for KSHV, EBV, and MHV68 (Projects 1, 2 and 3). The services of Core C will aid the overall goal of the program project to comprehensively analyze the role of herpesviral non-coding RNAs as well as studies on host cellular lncRNAs that are perturbed in response to viral infection. In addition to constructing recombinant viruses, Core C will perform genome-wide sequencing of viral mutants. Building upon years of experience in making a complete set of KSHV miRNA deletion mutants, our group has developed and implemented techniques for making bacmid-derived mutants in both KSHV and MHV68, and will readily extend this to EBV.

Public Health Relevance

The Virus Genomics Core provides an efficient, quality controlled, and cost-efficient research core that generates recombinant gamma-herpesviruses with defined mutations. This core will support the identification of lncRNA-dependent phenotypes for the oncogenic herpesviruses KSHV, EBV, and MHV68.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA214091-02
Application #
9429082
Study Section
Special Emphasis Panel (ZCA1)
Project Start
Project End
Budget Start
2018-02-01
Budget End
2019-01-31
Support Year
2
Fiscal Year
2018
Total Cost
Indirect Cost

  Institution

Name
University of Florida
Department
Type
DUNS #
969663814
City
Gainesville
State
FL
Country
United States
Zip Code
32611

  Publications

Van Skike, Nick D; Minkah, Nana K; Hogan, Chad H et al. (2018) Viral FGARAT ORF75A promotes early events in lytic infection and gammaherpesvirus pathogenesis in mice. PLoS Pathog 14:e1006843
Bullard, Whitney L; Flemington, Erik K; Renne, Rolf et al. (2018) Connivance, Complicity, or Collusion? The Role of Noncoding RNAs in Promoting Gammaherpesvirus Tumorigenesis. Trends Cancer 4:729-740
Sethuraman, Sunantha; Thomas, Merin; Gay, Lauren A et al. (2018) Computational analysis of ribonomics datasets identifies long non-coding RNA targets of ?-herpesviral miRNAs. Nucleic Acids Res 46:8574-8589
Jain, Vaibhav; Renne, Rolf (2018) Visualization of molecular biology: The LANA tether. Proc Natl Acad Sci U S A 115:4816-4818
Dai, Lu; Del Valle, Luis; Miley, Wendell et al. (2018) Transactivation of human endogenous retrovirus K (HERV-K) by KSHV promotes Kaposi's sarcoma development. Oncogene 37:4534-4545
Gay, Lauren A; Sethuraman, Sunantha; Thomas, Merin et al. (2018) Modified Cross-Linking, Ligation, and Sequencing of Hybrids (qCLASH) Identifies Kaposi's Sarcoma-Associated Herpesvirus MicroRNA Targets in Endothelial Cells. J Virol 92:
Mei, Suyu; Flemington, Erik K; Zhang, Kun (2018) Transferring knowledge of bacterial protein interaction networks to predict pathogen targeted human genes and immune signaling pathways: a case study on M. tuberculosis. BMC Genomics 19:505
Gay, Lauren A; Turner, Peter C; Renne, Rolf (2018) Contemporary Ribonomics Methods for Viral microRNA Target Analysis. Noncoding RNA 4:
Tonnessen-Murray, Crystal; Ungerleider, Nathan A; Rao, Sonia G et al. (2018) p53 Mediates Vast Gene Expression Changes That Contribute to Poor Chemotherapeutic Response in a Mouse Model of Breast Cancer. Transl Oncol 11:930-940
Zhang, Wensheng; Flemington, Erik K; Zhang, Kun (2018) Driver gene mutations based clustering of tumors: methods and applications. Bioinformatics 34:i404-i411

Showing the most recent 10 out of 27 publications