The purpose of the Recombinant Virus Core C is to generate quality controlled recombinant herpesviruses for KSHV, EBV, and MHV68 (Projects 1, 2 and 3). The services of Core C will aid the overall goal of the program project to comprehensively analyze the role of herpesviral non-coding RNAs as well as studies on host cellular lncRNAs that are perturbed in response to viral infection. In addition to constructing recombinant viruses, Core C will perform genome-wide sequencing of viral mutants. Building upon years of experience in making a complete set of KSHV miRNA deletion mutants, our group has developed and implemented techniques for making bacmid-derived mutants in both KSHV and MHV68, and will readily extend this to EBV.
The Virus Genomics Core provides an efficient, quality controlled, and cost-efficient research core that generates recombinant gamma-herpesviruses with defined mutations. This core will support the identification of lncRNA-dependent phenotypes for the oncogenic herpesviruses KSHV, EBV, and MHV68.
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|Zhang, Wensheng; Edwards, Andrea; Flemington, Erik K et al. (2017) Racial disparities in patient survival and tumor mutation burden, and the association between tumor mutation burden and cancer incidence rate. Sci Rep 7:13639|