Investigations into the molecular targets of drugs of abuse and their associated intracellular signaling pathways by this Program Project Grant have yielded a wealth of information regarding the cellular perturbations associated with these drugs. The combined studies outlined in Project 1-3 will extend upon this existing knowledge with rigorous cell biological, molecular, biochemical, behavioral and electrophysiological studies of striatal neurons following drug treatment. The Scientific Core will be devoted to facilitating the experiments proposed in Projects 1-3. The centralized responsibility for the performance of routine tasks, such as the maintenance of mouse colonies and the supply of common reagents, provides for an efficient, flexible and cost-effective means to ensure an adequate supply of required materials for all Projects.The major aims of the Scientific Core will be:
Aim I. The characterization and maintenance of transgenic mouse colonies;
Aim II. The production of key reagents, including polyclonal antibodies, phosphorylation state-specific antibodies, the design and execution of yeast two-hybrid screens, and the production of AAV viruses;
Aim III. The analysis of dendritic spines. All Scientific Core Aims will be conducted in close, ongoing consultation with the staff from Projects 1-3, such that services are delivered as required. The mice produced by the Scientific Core will be essential for many of the studies proposed in Projects 1-3, due to a heavy reliance on genetically modified animals. Production by a centralized Core will ensure optimal animal production and use. The biochemical and immunological reagents generated by the Scientific Core will also be necessary for Projects. The analysis of dendritic spines will be essential for Project 1, and will also help Projects 2 and 3 to carry out their proposed studies of dendritic spine morphology.
The proposed studies in this Program Project Grant will have the potential to provide greater insights into the causes of drug dependence as well as to help identify possible novel targets for pharmacological intervention. The Scientific Core will support this effort through facilitation of the three Projects in the Program Project Grant.
|Colangelo, Christopher M; Ivosev, Gordana; Chung, Lisa et al. (2015) Development of a highly automated and multiplexed targeted proteome pipeline and assay for 112 rat brain synaptic proteins. Proteomics 15:1202-14|
|Wan, Xun; Torregrossa, Mary M; Sanchez, Hayde et al. (2014) Activation of exchange protein activated by cAMP in the rat basolateral amygdala impairs reconsolidation of a memory associated with self-administered cocaine. PLoS One 9:e107359|
|Kitchen, Robert R; Rozowsky, Joel S; Gerstein, Mark B et al. (2014) Decoding neuroproteomics: integrating the genome, translatome and functional anatomy. Nat Neurosci 17:1491-9|
|Arango-Lievano, Margarita; Schwarz, Justin T; Vernov, Mary et al. (2014) Cell-type specific expression of p11 controls cocaine reward. Biol Psychiatry 76:794-801|
|Dupré, Aude; Daldello, Enrico M; Nairn, Angus C et al. (2014) Phosphorylation of ARPP19 by protein kinase A prevents meiosis resumption in Xenopus oocytes. Nat Commun 5:3318|
|Meyer, Douglas A; Torres-Altoro, Melissa I; Tan, Zhenjun et al. (2014) Ischemic stroke injury is mediated by aberrant Cdk5. J Neurosci 34:8259-67|
|Magupalli, Venkat G; Mochida, Sumiko; Yan, Jin et al. (2013) Ca2+-independent activation of Ca2+/calmodulin-dependent protein kinase II bound to the C-terminal domain of CaV2.1 calcium channels. J Biol Chem 288:4637-48|
|Oh, Yong-Seok; Gao, Pu; Lee, Ko-Woon et al. (2013) SMARCA3, a chromatin-remodeling factor, is required for p11-dependent antidepressant action. Cell 152:831-43|
|Yamagata, Yoko; Kaneko, Koichi; Kase, Daisuke et al. (2013) Regulation of ERK1/2 mitogen-activated protein kinase by NMDA-receptor-induced seizure activity in cortical slices. Brain Res 1507:1-10|
|Rebholz, Heike; Zhou, Mingming; Nairn, Angus C et al. (2013) Selective knockout of the casein kinase 2 in d1 medium spiny neurons controls dopaminergic function. Biol Psychiatry 74:113-21|
Showing the most recent 10 out of 179 publications