The goal of this program project is to fully characterize dental hard tissues, provide important information on their biomechanics and mineralization, and modify them to enhance adhesion of polymer based adhesives of them. This revised proposal is for years 11-15 of a program project grant, Characterization and Modification of Dental Hard Tissues. For ten years new information has been generated about the nature of the dentin substrate for preventive and restorative dentistry, including its structure, mechanical and etching properties, and its interface with polymeric dentin adhesives. In this proposed period the substrate will be expanded to include other forms of dentin, as well as enamel and cementum. The program project has a Laboratory, Statistical and Administrative Core and 4 projects: Structure/Properties of Altered Forms of Dentin and Cementum; Biomechanics of Dentin; The Bonding Zone with Dental Calcified Tissues; and Tools for Tissue Engineering Tooth Structure. The Core will coordinate all projects administratively, collect teeth and provide specimens for all experiments on all projects, manage the data base, conduct statistical analyses and insure that the results are disseminated. Project 8 will identify key materials structure/properties relationships that allow quantitative and unique identification of a broad array of various forms of dentin, its alterations due to age, disease processes and treatment and cementum. The new knowledge will serve as a foundation for improved understanding of these tissues and their variations as well as provide insights into dental calcified tissues that will be useful to advancing concepts is tissue engineering. The information also will be used to model mechanical properties of the tooth, including its fundamental elastic constants and factors contributing to fracture resistance and failure in one project. These models will be made on 3 length scales, from the constituent to the continuum. Thus projects 8 and 9 are complementary to each other in understanding dental calcified tissues. Project 10 will use the information from projects 8 and 9 to develop novel substrate morphologies and properties that will enhance the durability of bonds to resin based composites. Project 11 will use the same methods as the other projects to evaluate transgenic mouse models for tissue engineering of teeth. It will focus on the analysis of dentin and cementum in the mouse model, and be used as a tool to determine the role of TGF-Beta on the development and repair of these tissues.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Program Projects (P01)
Project #
5P01DE009859-14
Application #
6921921
Study Section
Special Emphasis Panel (ZDE1-PW (03))
Program Officer
Hunziker, Rosemarie
Project Start
1991-08-01
Project End
2007-06-30
Budget Start
2005-07-01
Budget End
2006-06-30
Support Year
14
Fiscal Year
2005
Total Cost
$1,176,931
Indirect Cost
Name
University of California San Francisco
Department
Dentistry
Type
Schools of Dentistry
DUNS #
094878337
City
San Francisco
State
CA
Country
United States
Zip Code
94143
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Chang, Jolie L; Brauer, Delia S; Johnson, Jacob et al. (2010) Tissue-specific calibration of extracellular matrix material properties by transforming growth factor-? and Runx2 in bone is required for hearing. EMBO Rep 11:765-71
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Hsu, Kuang-Wei; Marshall, Sally J; Pinzon, Lilliam M et al. (2008) SEM evaluation of resin-carious dentin interfaces formed by two dentin adhesive systems. Dent Mater 24:880-7
Koester, Kurt J; Ager 3rd, Joel W; Ritchie, Robert O (2008) The effect of aging on crack-growth resistance and toughening mechanisms in human dentin. Biomaterials 29:1318-28

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