The main goal of the Cell Culture Core facility is to provide a stable, reproducible and reliable source of virus stocks and relevant KSHV-infected and uninfected primary and immortalized cell cultures for all of the projects involved in this program. This centralized source would be cost-saving and provide for reproducible experimentation across the different laboratories. The Core will support all four projects with the following aims 1) produce, isolate and titer stocks of KSHV and recombinant KSHV strains 2) grow, maintain and provide KSHV-infected and noninfected tissue culture cells 3) process tonsil and oral KS biopsies for tissue sectioning or primary cultures 4) provide training for virus production and purification, cell culture growth and maintenance and for biosafety methods and procedures for producing and utilizing herpesviruses in research.

Public Health Relevance

The Kaposi's sarcoma (KS)-associated herpesvirus (KSHV) is the cause of KS and two other AIDS-related malignancies. KS is the most common oral tumor associated with HIV/AIDS and is one of the most common pediatric and adult tumors in Sub-Saharan Africa. This project will investigate the immune control, activation, transmission and pathogenesis of KSHV in order to develop effective vaccines and therapeutics for this devastatino viral pathogen.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Program Projects (P01)
Project #
5P01DE021954-04
Application #
8657379
Study Section
Special Emphasis Panel (ZDE1)
Project Start
Project End
Budget Start
2014-05-01
Budget End
2015-04-30
Support Year
4
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Seattle Children's Hospital
Department
Type
DUNS #
City
Seattle
State
WA
Country
United States
Zip Code
98121
Ikoma, Minako; Gantt, Soren; Casper, Corey et al. (2018) KSHV oral shedding and plasma viremia result in significant changes in the extracellular tumorigenic miRNA expression profile in individuals infected with the malaria parasite. PLoS One 13:e0192659
Garrigues, H Jacques; Howard, Kellie; Barcy, Serge et al. (2017) Full-Length Isoforms of Kaposi's Sarcoma-Associated Herpesvirus Latency-Associated Nuclear Antigen Accumulate in the Cytoplasm of Cells Undergoing the Lytic Cycle of Replication. J Virol 91:
Bruce, A Gregory; Barcy, Serge; DiMaio, Terri et al. (2017) Quantitative Analysis of the KSHV Transcriptome Following Primary Infection of Blood and Lymphatic Endothelial Cells. Pathogens 6:
Sychev, Zoi E; Hu, Alex; DiMaio, Terri A et al. (2017) Integrated systems biology analysis of KSHV latent infection reveals viral induction and reliance on peroxisome mediated lipid metabolism. PLoS Pathog 13:e1006256
Sanchez, Erica L; Pulliam, Thomas H; Dimaio, Terri A et al. (2017) Glycolysis, Glutaminolysis, and Fatty Acid Synthesis Are Required for Distinct Stages of Kaposi's Sarcoma-Associated Herpesvirus Lytic Replication. J Virol 91:
DiMaio, Terri A; Wentz, Breanna L; Lagunoff, Michael (2016) Isolation and characterization of circulating lymphatic endothelial colony forming cells. Exp Cell Res 340:159-69
Bruce, A Gregory; Horst, Jeremy A; Rose, Timothy M (2016) Conservation of the glycoprotein B homologs of the Kaposi?s sarcoma-associated herpesvirus (KSHV/HHV8) and old world primate rhadinoviruses of chimpanzees and macaques. Virology 494:29-46
Lagunoff, Michael (2016) Activation of cellular metabolism during latent Kaposi's Sarcoma herpesvirus infection. Curr Opin Virol 19:45-9
Sanchez, Erica L; Carroll, Patrick A; Thalhofer, Angel B et al. (2015) Latent KSHV Infected Endothelial Cells Are Glutamine Addicted and Require Glutaminolysis for Survival. PLoS Pathog 11:e1005052
Sanchez, Erica L; Lagunoff, Michael (2015) Viral activation of cellular metabolism. Virology 479-480:609-18

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