Abstract

The Protein Chemistry Core is designed to optimize the efficiency and most cost effectiveness of the Program Project by performing laboratory procedures used in common by the Project Investigators. The major focus of the Core will be the use of gas chromatography-mass spectrometry to determine the levels of glycation products and oxidation products in proteins, cells and tissues used in the various Projects. Specific marker for proteins damaged by glycation, glycoxidation, the myeloperoxidase system of activated phagocytes, and reactive nitrogen species will be quantified using stable isotope dilution, negative-ion chemical ionization gas chromatography-mass spectrometry. By centralizing and standardizing procedures, the Core facility will provide a common set of analytical procedures that should facilitate a unified understanding of the molecular mechanisms involved in the genesis of diabetic vascular disease.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
5P01DK002456-42
Application #
6418179
Study Section
Project Start
2000-12-01
Project End
2002-03-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
42
Fiscal Year
2001
Total Cost
$130,479
Indirect Cost

  Institution

Name
University of Washington
Department
Type
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195

  Publications

Hutchins, Patrick M; Heinecke, Jay W (2015) Cholesterol efflux capacity, macrophage reverse cholesterol transport and cardioprotective HDL. Curr Opin Lipidol 26:388-93
Vaisar, Tomáš; Tang, Chongren; Babenko, Ilona et al. (2015) Inflammatory remodeling of the HDL proteome impairs cholesterol efflux capacity. J Lipid Res 56:1519-30
Hutchins, Patrick M; Ronsein, Graziella E; Monette, Jeffrey S et al. (2014) Quantification of HDL particle concentration by calibrated ion mobility analysis. Clin Chem 60:1393-401
Purnell, Jonathan Q; Zinman, Bernard; Brunzell, John D et al. (2013) The effect of excess weight gain with intensive diabetes mellitus treatment on cardiovascular disease risk factors and atherosclerosis in type 1 diabetes mellitus: results from the Diabetes Control and Complications Trial/Epidemiology of Diabetes Interven Circulation 127:180-7
Umemoto, Tomio; Han, Chang Yeop; Mitra, Poulami et al. (2013) Apolipoprotein AI and high-density lipoprotein have anti-inflammatory effects on adipocytes via cholesterol transporters: ATP-binding cassette A-1, ATP-binding cassette G-1, and scavenger receptor B-1. Circ Res 112:1345-54
Liu, Yuhua; Tang, Chongren (2012) Regulation of ABCA1 functions by signaling pathways. Biochim Biophys Acta 1821:522-9
de Boer, Ian H; Sachs, Michael C; Cleary, Patricia A et al. (2012) Circulating vitamin D metabolites and kidney disease in type 1 diabetes. J Clin Endocrinol Metab 97:4780-8
Hoofnagle, Andrew N; Wu, Mingyuan; Gosmanova, Albina K et al. (2010) Low clusterin levels in high-density lipoprotein associate with insulin resistance, obesity, and dyslipoproteinemia. Arterioscler Thromb Vasc Biol 30:2528-34
Tang, Chongren; Kanter, Jenny E; Bornfeldt, Karin E et al. (2010) Diabetes reduces the cholesterol exporter ABCA1 in mouse macrophages and kidneys. J Lipid Res 51:1719-28
Vaisar, Tomás; Kassim, Sean Y; Gomez, Ivan G et al. (2009) MMP-9 sheds the beta2 integrin subunit (CD18) from macrophages. Mol Cell Proteomics 8:1044-60

Showing the most recent 10 out of 24 publications