(instructions): The Histopathology Core (Core B) is an established Core facility of the Program Project that has the overall goal to enhance and facilitate the research of the individual Research Units by providing the following histological services to Program members: 1. Fixation, processing, embedding, and storage of tissues a. Assistance with tissue collection and fixation b. Processing and embedding in paraffin c. Freezing of tissues d. Storage of fixed and processed specimens 2. Tissue sectioning a. Preparation of paraffin sections b. Preparation of frozen and live tissue sections 3. Standard and immunohistological staining procedures a. Standard and specialized histochemical staining b. Immunohistological staining with enzyme- or fluorochrome-labeled antibodies c. Antigen retrieval and signal amplification methods d. TUNEL and BrdU assays for detection of apoptotic and proliferating cells 4. Assistance with design, microscopic analysis, and data interpretation of histological studies a. Advice on experimental design of histological studies b. Assistance with microscopic analysis, histological evaluation, and photodocumentation c. Training in tissue collection, staining methods, and histological interpretation The Core is fully equipped and has highly experienced personnel to provide these services at the highest technical quality in a cost-effective manner.
Proper evaluation of the complex cellular and molecular events that occur in the intestinal tract and beyond under resting and challenge conditions requires extensive histological analysis. The successful conduct of histological studies depends on an equipment infrastructure and expertise that are unique to the field, and are best provided through a dedicated Core service arrangement.
|Bertin, S; Lozano-Ruiz, B; Bachiller, V et al. (2015) Dual-specificity phosphatase 6 regulates CD4+ T-cell functions and restrains spontaneous colitis in IL-10-deficient mice. Mucosal Immunol 8:505-15|
|Vicente-Suarez, I; Larange, A; Reardon, C et al. (2015) Unique lamina propria stromal cells imprint the functional phenotype of mucosal dendritic cells. Mucosal Immunol 8:141-51|
|Spatola, B N; Kaukinen, K; Collin, P et al. (2014) Persistence of elevated deamidated gliadin peptide antibodies on a gluten-free diet indicates nonresponsive coeliac disease. Aliment Pharmacol Ther 39:407-17|
|Debnath, Anjan; Shahinas, Dea; Bryant, Clifford et al. (2014) Hsp90 inhibitors as new leads to target parasitic diarrheal diseases. Antimicrob Agents Chemother 58:4138-44|
|Dann, Sara M; Le, Christine; Choudhury, Barun K et al. (2014) Attenuation of intestinal inflammation in interleukin-10-deficient mice infected with Citrobacter rodentium. Infect Immun 82:1949-58|
|Meehan, T F; Witherden, D A; Kim, C-H et al. (2014) Protection against colitis by CD100-dependent modulation of intraepithelial ?? T lymphocyte function. Mucosal Immunol 7:134-42|
|Bertin, Samuel; Aoki-Nonaka, Yukari; de Jong, Petrus Rudolf et al. (2014) The ion channel TRPV1 regulates the activation and proinflammatory properties of CD4? T cells. Nat Immunol 15:1055-63|
|de Jong, Petrus R; Takahashi, Naoki; Harris, Alexandra R et al. (2014) Ion channel TRPV1-dependent activation of PTP1B suppresses EGFR-associated intestinal tumorigenesis. J Clin Invest 124:3793-806|
|González-Navajas, José M; Corr, Mary P; Raz, Eyal (2014) The immediate protective response to microbial challenge. Eur J Immunol 44:2536-49|
|Barbero, Erika M; McNally, Shawna L; Donohue, Michael C et al. (2014) Barriers impeding serologic screening for celiac disease in clinically high-prevalence populations. BMC Gastroenterol 14:42|
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