Core B has continuously served all projects of the PPG for the past 23 years. The roles of this core are divided into two divisions. Division I oversees the acquisition, breeding and maintenance of mice colonies, the predominant species used for this project. It will also breed new inducible transgenic mouse strains using the Cre-Lox system to produce cell specific gene modifications that are optimally suited to address the aims ofthe various projects. Division I will also provide freshly dispersed cells for investigators as well as cultured cells and tissues. The PPG has been extremely fortunate to have a long standing arrangement with Charies River Laboratories which provides us with gastrointestinal muscles from the Cynomolgus monkey. Core B is responsible for the acquisition and distribution of these tissues to investigators as well obtaining muscle samples from patients undergoing surgery for diseases of the colon. The role of Division II is to oversee the flow cytometry activities of Core B. This division has been extremely successful in using flow activated cell sorting (FACS) to separate and analyze select populations of cells isolated from the tunica muscularis. The acquisition of transgenic mice expressing GFP in smooth muscle cells (smMHCCre-egfp), ICC (KitcopGFP/-!-) or PDGFRa+ cells (PDGFRa-egfp/+) has further advanced our ability to sort and analyze highly pure populations of cells in sufficient quantities to create high resolution gene expression profiles on a genome-wide scale. Generating these cell specific transcriptomes will allow investigators to compare gene expression in different cell types and within a given cell type with development or during a pathological condition such as partial obstruction. In summary. Core B is an indispensable and highly successful component ofthe PPG that continues to grow and diversify as new methodologies and approaches become available.

Public Health Relevance

This study will provide important new information with regard to how motility in the intestine is controlled. Although gastrointestinal disorders usually do not kill patients they result in enormous pain and suffering. The treatments available are rarely sufficient and require billions of health care dollars. The studies described here are highly innovative and will aid in developing new treatments for Gl disorders.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
2P01DK041315-26
Application #
8742144
Study Section
Special Emphasis Panel (ZDK1-GRB-6 (J3))
Project Start
Project End
Budget Start
2014-08-20
Budget End
2015-07-31
Support Year
26
Fiscal Year
2014
Total Cost
$278,987
Indirect Cost
$84,571
Name
University of Nevada Reno
Department
Type
DUNS #
146515460
City
Reno
State
NV
Country
United States
Zip Code
89557
Baker, Salah A; Drumm, Bernard T; Saur, Dieter et al. (2016) Spontaneous Ca(2+) transients in interstitial cells of Cajal located within the deep muscular plexus of the murine small intestine. J Physiol 594:3317-38
Durnin, Leonie; Hayoz, Sebastien; Corrigan, Robert D et al. (2016) Urothelial purine release during filling of murine and primate bladders. Am J Physiol Renal Physiol 311:F708-F716
Sanders, Kenton M; Ward, Sean M; Friebe, Andreas (2016) Rebuttal from Kenton M. Sanders, Sean M. Ward and Andreas Friebe. J Physiol 594:1515
Hwang, Sung Jin; Basma, Naseer; Sanders, Kenton M et al. (2016) Effects of new-generation inhibitors of the calcium-activated chloride channel anoctamin 1 on slow waves in the gastrointestinal tract. Br J Pharmacol 173:1339-49
Durnin, L; Moreland, N; Lees, A et al. (2016) A commonly used ecto-ATPase inhibitor, ARL-67156, blocks degradation of ADP more than the degradation of ATP in murine colon. Neurogastroenterol Motil 28:1370-81
Sanders, Kenton M; Ward, Sean M; Friebe, Andreas (2016) CrossTalk proposal: Interstitial cells are involved and physiologically important in neuromuscular transmission in the gut. J Physiol 594:1507-9
Sanders, Kenton M (2015) New Molecular Tools to Investigate the Development and Functions of Interstitial Cells of Cajal in the GI Tract. Gastroenterology 149:283-6
Peri, Lauren E; Koh, Byoung H; Ward, Grace K et al. (2015) A novel class of interstitial cells in the mouse and monkey female reproductive tracts. Biol Reprod 92:102
Baker, Salah A; Hennig, Grant W; Ward, Sean M et al. (2015) Temporal sequence of activation of cells involved in purinergic neurotransmission in the colon. J Physiol 593:1945-63
Zhu, Mei Hong; Sung, Tae Sik; O'Driscoll, Kate et al. (2015) Intracellular Ca(2+) release from endoplasmic reticulum regulates slow wave currents and pacemaker activity of interstitial cells of Cajal. Am J Physiol Cell Physiol 308:C608-20

Showing the most recent 10 out of 349 publications