Abstract

Core B will provide expertise and contain instrumentation for the highly sensitive analyses of proteins and peptides, and for large scale and high resolution genetic mapping and DNA sequencing. Core B will focus on aiding the investigators of Projects 1-4 with analyses of proteins, peptides, genes and genetic traits. The Li-Cor model 4000L infrared DNA sequencer configurable for a variety of procedures for gel electrophoresis and with a powerful laser detection system will be used for DNA sequencing and genetic mapping. In addition, standard mapping techniques will be employed for candidate IDDM and NIDDM genes. Equipment available for sequence analysis of proteins an Class II peptides include a tandem mass spectrometer (TSQ700) equipped with electrospray ionization and a laser desorption time-of-flight mass spectrometer. Techniques are established to analyze peptides bound to Class II MHC molecules, to examine conditions for correct antigen presentation as well as to analyze antigen processing in subcellular vesicles. Core B facility should have an enormous throughput for DNA sequencing and genetic mapping.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
5P01DK053004-03
Application #
6301170
Study Section
Project Start
1999-12-01
Project End
2000-11-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
3
Fiscal Year
2000
Total Cost
$179,118
Indirect Cost

  Institution

Name
University of Washington
Department
Type
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195

  Publications

Zhao, Lue Ping; Bolouri, Hamid; Zhao, Michael et al. (2016) An Object-Oriented Regression for Building Disease Predictive Models with Multiallelic HLA Genes. Genet Epidemiol 40:315-32
Hao, Wei; Greenbaum, Carla J; Krischer, Jeffrey P et al. (2015) The Effect of DPT-1 Intravenous Insulin Infusion and Daily Subcutaneous Insulin on Endogenous Insulin Secretion and Postprandial Glucose Tolerance. Diabetes Care 38:891-6
Brooks-Worrell, Barbara M; Boyko, Edward J; Palmer, Jerry P (2014) Impact of islet autoimmunity on the progressive ?-cell functional decline in type 2 diabetes. Diabetes Care 37:3286-93
Eringsmark Regnéll, S; Lernmark, A (2013) The environment and the origins of islet autoimmunity and Type 1 diabetes. Diabet Med 30:155-60
Brooks-Worrell, B M; Palmer, J P (2013) Attenuation of islet-specific T cell responses is associated with C-peptide improvement in autoimmune type 2 diabetes patients. Clin Exp Immunol 171:164-70
Skoglund, Camilla; Chéramy, Mikael; Casas, Rosaura et al. (2012) GAD autoantibody epitope pattern after GAD-alum treatment in children and adolescents with type 1 diabetes. Pediatr Diabetes 13:244-50
Nepom, Gerald T (2012) MHC class II tetramers. J Immunol 188:2477-82
Oak, S; Radtke, J; Torn, C et al. (2011) Immunoglobulin subclass profiles of anti-idiotypic antibodies to GAD65Ab differ between type 1 diabetes patients and healthy individuals. Scand J Immunol 74:363-7
Brooks-Worrell, Barbara M; Reichow, Jessica L; Goel, Amit et al. (2011) Identification of autoantibody-negative autoimmune type 2 diabetic patients. Diabetes Care 34:168-73
Vaziri-Sani, Fariba; Oak, Shilpa; Radtke, Jared et al. (2010) ZnT8 autoantibody titers in type 1 diabetes patients decline rapidly after clinical onset. Autoimmunity 43:598-606

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