Imaging Core B will continue to serve as an integrating center for all image-based techniques, procedures and analyses needed and used by all Projects in this Program Project. Core B will facilitate and coordinate technical interactions among the Projects and the Cores. Imaging Core B will provide the technical expertise, experience, computers, software, image acquisition, image processing and quantitative image analysis methods required to fully support the image-related research proposed in all three projects in this Program Project. This will be carried out by three distinct but related and synergistic components, namely In Vitro and Small Animal Imaging, In Vivo Human Imaging and Quantitative Image Analysis. All in vitro and in vivo image data produced by the three Projects wall be managed by Core B, from acquisition to analysis to reporting. Core B will provide routine and common services, such as data storage and backup, specimen preparation and imaging, 3D image reconstruction and rendering, robust image processing and quantitative analyses, database management, results reporting and external website maintenance. Several new methods will be developed for the projects - automated feature extraction, counting and analysis in cell and molecular images, dynamic MR imaging of the gastro-intestinal tract in patients, and advanced 3D image enhancement, segmentation, registration, classification and fusion of multimodality images of small animals. The Imaging Core will provide an efficient repository for all images and analysis results organized into a relational database for ready access by all Project investigators. Small animal image analysis results will be exported to the Core C database (EARR) for association with physiological data. Core B and C will together develop integrated data management tools to transparently access these two databases. These database management tools will facilitate comparative analyses and comprehensive data mining to determine associations and patterns in multimodality and longitudinal (temporal) datasets within and across projects. A website containing background, methods, results, publications and other relevant topics associated with all projects and cores in the Program Project will be maintained and routinely updated by Core B for external sharing of information produced for and by the Program Project.

Public Health Relevance

This Program Project is addressing critical unsolved problems in identification and characterization of dysregulation of motility in the gastrointestinal tract of patients with diabetes and eating disorders. The imaging science employed at both organ and cellular levels and the quantitative image analysis methods developed in this Core project will significantly facilitate achievement of the specific aims of all research in this program, leading to more comprehensive diagnoses and effective therapies for these disorders.

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Research Program Projects (P01)
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Special Emphasis Panel (ZDK1-GRB-9 (J1))
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Mayo Clinic, Rochester
United States
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Cipriani, Gianluca; Gibbons, Simon J; Verhulst, Pieter-Jan et al. (2016) Diabetic Csf1(op/op) mice lacking macrophages are protected against the development of delayed gastric emptying. Cell Mol Gastroenterol Hepatol 2:40-47
Rajan, Elizabeth; Gostout, Christopher J; Wong Kee Song, Louis M et al. (2016) Innovative gastric endoscopic muscle biopsy to identify all cell types, including myenteric neurons and interstitial cells of Cajal in patients with idiopathic gastroparesis: a feasibility study (with video). Gastrointest Endosc 84:512-7
Cipriani, Gianluca; Gibbons, Simon J; Kashyap, Purna C et al. (2016) Intrinsic Gastrointestinal Macrophages: Their Phenotype and Role in Gastrointestinal Motility. Cell Mol Gastroenterol Hepatol 2:120-130.e1
Nelson, A D; Camilleri, M; Acosta, A et al. (2016) Effects of ghrelin receptor agonist, relamorelin, on gastric motor functions and satiation in healthy volunteers. Neurogastroenterol Motil 28:1705-1713
Wang, Zhiquan; Zhang, Honglian; Liu, Ji et al. (2016) USP51 deubiquitylates H2AK13,15ub and regulates DNA damage response. Genes Dev 30:946-59
Yan, Huihuang; Tian, Shulan; Slager, Susan L et al. (2016) Genome-Wide Epigenetic Studies in Human Disease: A Primer on -Omic Technologies. Am J Epidemiol 183:96-109
Choi, Kyoung Moo; Gibbons, Simon J; Sha, Lei et al. (2016) Interleukin 10 Restores Gastric Emptying, Electrical Activity, and Interstitial Cells of Cajal Networks in Diabetic Mice. Cell Mol Gastroenterol Hepatol 2:454-467
Eisenman, S T; Gibbons, S J; Verhulst, P-J et al. (2016) Tumor necrosis factor alpha derived from classically activated ""M1"" macrophages reduces interstitial cell of Cajal numbers. Neurogastroenterol Motil :
Bharucha, A E; Daley, S L; Low, P A et al. (2016) Effects of hemin on heme oxygenase-1, gastric emptying, and symptoms in diabetic gastroparesis. Neurogastroenterol Motil 28:1731-1740
Halland, Magnus; Bharucha, Adil E (2016) Relationship Between Control of Glycemia and Gastric Emptying Disturbances in Diabetes Mellitus. Clin Gastroenterol Hepatol 14:929-36

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