The Mouse/Histology Core B will provide SAMPI/YitFc (SAMP) mice and other mouse modesi of experimental CD for use in each of the individual projects. Maintaining the SAMP breeding colony and experimental animals in a common centralized facility under uniform environmental conditions is crucial to the success of the program. Centralization of mouse production will also reduce costs by eliminating redundant breeding colonies, and ulfimately reduce the numbers of mice required by ensuring efficient utilization by each of the projects.
A second aim i s to provide centralized histological services and assistance with immunohistochemistry, digital fluorescence and brightfield microscopy for each of the projects.
The third aim of the Core is to provide centralized histopathological analyses of inflammation and other morphological parameters to invesfigators within the program. A centralized core component for analysis of the histopathological features of ileitis and colitis in this model will provide a common framework for interpretation of data generated by each of the four projects. A strength of this component is the availability of an experienced Gl pathologists. Dr. Xin, to perform histopathological scoring of samples to each of the projects and to provide ongoing consultative service to the projects conceming the pathological features of disease in this model. The forth aim is to provide consultation and teaching on immunohistochemical techniques and marker-assisted breed strategies for the generation of mutant congenic mouse strains that will be useful to projects within the program. Transfer of these induced mutations into an appropriate background can be accomplished in 5 generations. Dr. Pizarro will direct the core. A committee consisting of the core director and project leaders, will address routine quality control and prioritization issues. An oversight committee will review the operation of the core on a yearly basis.

Public Health Relevance

CD affects more than 500,000 individuals in the US and incurs significant costs to society. Understanding the precise mechanisms and immune defects that cause the disease will allow us to develop better therapies and begin to develop a cure for this devastating disease.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
5P01DK091222-02
Application #
8381638
Study Section
Special Emphasis Panel (ZDK1-GRB-6)
Project Start
Project End
Budget Start
2012-08-01
Budget End
2013-07-31
Support Year
2
Fiscal Year
2012
Total Cost
$175,183
Indirect Cost
$72,939
Name
Case Western Reserve University
Department
Type
DUNS #
077758407
City
Cleveland
State
OH
Country
United States
Zip Code
44106
Kumar, Anand; Davenport, Karen Walston; Vuyisich, Grace et al. (2018) Complete Genome Sequences of Historic Clostridioides difficile Food-Dwelling Ribotype 078 Strains in Canada Identical to That of the Historic Human Clinical Strain M120 in the United Kingdom. Microbiol Resour Announc 7:
Rathkey, Joseph K; Zhao, Junjie; Liu, Zhonghua et al. (2018) Chemical disruption of the pyroptotic pore-forming protein gasdermin D inhibits inflammatory cell death and sepsis. Sci Immunol 3:
Mehta, Kathan; Jaiswal, Palashkumar; Briggs, Farren et al. (2018) In-patient outcomes of Hematopoietic Stem Cell Transplantation in Patients with Immune Mediated Inflammatory Diseases: A Nationwide Study. Sci Rep 8:6825
Yang, Jie; Liu, Zhonghua; Wang, Chuanping et al. (2018) Mechanism of gasdermin D recognition by inflammatory caspases and their inhibition by a gasdermin D-derived peptide inhibitor. Proc Natl Acad Sci U S A 115:6792-6797
Liu, Zhonghua; Wang, Chuanping; Rathkey, Joseph K et al. (2018) Structures of the Gasdermin D C-Terminal Domains Reveal Mechanisms of Autoinhibition. Structure 26:778-784.e3
Perez, Jessica M; Chen, Yinghua; Xiao, Tsan S et al. (2018) Phosphorylation of the E3 ubiquitin protein ligase ITCH diminishes binding to its cognate E2 ubiquitin ligase. J Biol Chem 293:1100-1105
Rodriguez-Palacios, Alexander; Aladyshkina, Natalia; Ezeji, Jessica C et al. (2018) 'Cyclical Bias' in Microbiome Research Revealed by A Portable Germ-Free Housing System Using Nested Isolation. Sci Rep 8:3801
Chirieleison, Steven M; Rathkey, Joseph K; Abbott, Derek W (2018) Unique BIR domain sets determine inhibitor of apoptosis protein-driven cell death and NOD2 complex signal specificity. Sci Signal 11:
Goodman, Wendy A; Havran, Hannah L; Quereshy, Humzah A et al. (2018) Estrogen Receptor ? Loss-of-Function Protects Female Mice From DSS-Induced Experimental Colitis. Cell Mol Gastroenterol Hepatol 5:630-633.e1
Li, Zhaodong; Buttó, Ludovica F; Buela, Kristine-Anne et al. (2018) Death Receptor 3 Signaling Controls the Balance between Regulatory and Effector Lymphocytes in SAMP1/YitFc Mice with Crohn's Disease-Like Ileitis. Front Immunol 9:362

Showing the most recent 10 out of 90 publications