Abstract

The successful achievement of the aims of this Program Project is crucially dependent on the support to be obtained from the Core. The provision of trouble-free computer administration and NMR spectrometer maintenance is incredibly important. A majority of the component projects, all of the Scripps projects, rely heavily on the Core. The commitment on the part of the Scripps Research Institute to the establishment and maintenance of state-of-the-art computing and NMR facilities has been reflected in the composition of the faculty which comprise a powerful and concentrated group of computational and structural scientists. The Core will provide the critical shared resources needed to maintain the NMR spectrometers and high-performance computers for the use of the investigators in this Program Project.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
5P01GM056879-02
Application #
6107839
Study Section
Project Start
1999-01-01
Project End
1999-12-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
2
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
Scripps Research Institute
Department
Type
DUNS #
City
La Jolla
State
CA
Country
United States
Zip Code
92037

  Publications

Boehr, David D; Dyson, H Jane; Wright, Peter E (2006) An NMR perspective on enzyme dynamics. Chem Rev 106:3055-79
Boehr, David D; McElheny, Dan; Dyson, H Jane et al. (2006) The dynamic energy landscape of dihydrofolate reductase catalysis. Science 313:1638-42
Thorpe, Ian F; Brooks 3rd, Charles L (2005) Conformational substates modulate hydride transfer in dihydrofolate reductase. J Am Chem Soc 127:12997-3006
McElheny, Dan; Schnell, Jason R; Lansing, Jonathan C et al. (2005) Defining the role of active-site loop fluctuations in dihydrofolate reductase catalysis. Proc Natl Acad Sci U S A 102:5032-7
Venkitakrishnan, Rani P; Zaborowski, Eduardo; McElheny, Dan et al. (2004) Conformational changes in the active site loops of dihydrofolate reductase during the catalytic cycle. Biochemistry 43:16046-55
Chen, Jianhan; Brooks 3rd, Charles L; Wright, Peter E (2004) Model-free analysis of protein dynamics: assessment of accuracy and model selection protocols based on molecular dynamics simulation. J Biomol NMR 29:243-57
Schnell, Jason R; Dyson, H Jane; Wright, Peter E (2004) Structure, dynamics, and catalytic function of dihydrofolate reductase. Annu Rev Biophys Biomol Struct 33:119-40
Schnell, Jason R; Dyson, H Jane; Wright, Peter E (2004) Effect of cofactor binding and loop conformation on side chain methyl dynamics in dihydrofolate reductase. Biochemistry 43:374-83
Thorpe, Ian F; Brooks 3rd, Charles L (2004) The coupling of structural fluctuations to hydride transfer in dihydrofolate reductase. Proteins 57:444-57
Osborne, Michael J; Venkitakrishnan, Rani P; Dyson, H Jane et al. (2003) Diagnostic chemical shift markers for loop conformation and substrate and cofactor binding in dihydrofolate reductase complexes. Protein Sci 12:2230-8

Showing the most recent 10 out of 28 publications