This Biochemical and Molecular Core facility, under the direction of Dr. David Harder with the assistance of Jayashree Narayanan, will provide a centralized professional service to all three projects involved in this Program. The staff will be trained by the project leaders or specialized personnel to perform a variety of procedures required in this Program Project Grant. This facility will maintain complete, computerized and centralized records and well-defined quality control guidelines for all assays. In addition new assays will be developed by Dr. Harder in consultation with investigators to meet the changing needs and directions of the Program. New assays that have thus far been developed by the Core include siRNA technology to silence gene expression, real time PCR to quantitate mRNA levels, metabolic models to record endothelial dysfunction and animal models to measure myocardial infarction. Dr. Harder and Ms. Narayanan have first hand experience in running core laboratories for two other Program Projects. The Core facility is located in the Cardiovascular Center which is in close proximity to the laboratories of the investigators for all three projects.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
5P01GM066730-10
Application #
8379752
Study Section
Special Emphasis Panel (ZGM1-PPBC-0)
Project Start
Project End
2014-07-31
Budget Start
2012-08-01
Budget End
2013-07-31
Support Year
10
Fiscal Year
2012
Total Cost
$380,446
Indirect Cost
$149,814
Name
Medical College of Wisconsin
Department
Type
DUNS #
937639060
City
Milwaukee
State
WI
Country
United States
Zip Code
53226
Bosnjak, Zeljko J; Ge, Zhi-Dong (2017) The application of remote ischemic conditioning in cardiac surgery. F1000Res 6:928
Liu, Yanan; Baumgardt, Shelley L; Fang, Juan et al. (2017) Transgenic overexpression of GTP cyclohydrolase 1 in cardiomyocytes ameliorates post-infarction cardiac remodeling. Sci Rep 7:3093
Sedlic, Filip; Muravyeva, Maria Y; Sepac, Ana et al. (2017) Targeted Modification of Mitochondrial ROS Production Converts High Glucose-Induced Cytotoxicity to Cytoprotection: Effects on Anesthetic Preconditioning. J Cell Physiol 232:216-24
Mattson, David L; Liang, Mingyu (2017) Hypertension: From GWAS to functional genomics-based precision medicine. Nat Rev Nephrol 13:195-196
Stowe, David F; Yang, Meiying; Heisner, James S et al. (2017) Endogenous and Agonist-induced Opening of Mitochondrial Big Versus Small Ca2+-sensitive K+ Channels on Cardiac Cell and Mitochondrial Protection. J Cardiovasc Pharmacol 70:314-328
Chuppa, Sandra; Liang, Mingyu; Liu, Pengyuan et al. (2017) MicroRNA-21 regulates peroxisome proliferator-activated receptor alpha, a molecular mechanism of cardiac pathology in Cardiorenal Syndrome Type 4. Kidney Int :
Camara, Amadou K S; Zhou, YiFan; Wen, Po-Chao et al. (2017) Mitochondrial VDAC1: A Key Gatekeeper as Potential Therapeutic Target. Front Physiol 8:460
Baker, Maria Angeles; Davis, Seth J; Liu, Pengyuan et al. (2017) Tissue-Specific MicroRNA Expression Patterns in Four Types of Kidney Disease. J Am Soc Nephrol 28:2985-2992
Liu, Yanan; Yan, Yasheng; Inagaki, Yasuyoshi et al. (2017) Insufficient Astrocyte-Derived Brain-Derived Neurotrophic Factor Contributes to Propofol-Induced Neuron Death Through Akt/Glycogen Synthase Kinase 3?/Mitochondrial Fission Pathway. Anesth Analg 125:241-254
Yang, MeiYing; Camara, Amadou K S; Aldakkak, Mohammed et al. (2017) Identity and function of a cardiac mitochondrial small conductance Ca2+-activated K+ channel splice variant. Biochim Biophys Acta 1858:442-458

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