Projects 1, 2 and 3 will require the use of NMR and EPR methodologies provided by Core B to investigate the structures of SNAREs and synaptotagmin and their interactions in micellar and membrane environments.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
5P01GM072694-07
Application #
8376202
Study Section
Special Emphasis Panel (ZRG1-BCMB-S)
Project Start
Project End
Budget Start
2012-07-01
Budget End
2013-06-30
Support Year
7
Fiscal Year
2012
Total Cost
$91,407
Indirect Cost
$32,052
Name
University of Virginia
Department
Type
DUNS #
065391526
City
Charlottesville
State
VA
Country
United States
Zip Code
22904
Witkowska, Agata; Jahn, Reinhard (2017) Rapid SNARE-Mediated Fusion of Liposomes and Chromaffin Granules with Giant Unilamellar Vesicles. Biophys J 113:1251-1259
Kreutzberger, Alex J B; Kiessling, Volker; Liang, Binyong et al. (2017) Asymmetric Phosphatidylethanolamine Distribution Controls Fusion Pore Lifetime and Probability. Biophys J 113:1912-1915
Katti, Sachin; Nyenhuis, Sarah B; Her, Bin et al. (2017) Non-Native Metal Ion Reveals the Role of Electrostatics in Synaptotagmin 1-Membrane Interactions. Biochemistry 56:3283-3295
Kreutzberger, Alex J B; Kiessling, Volker; Liang, Binyong et al. (2017) Reconstitution of calcium-mediated exocytosis of dense-core vesicles. Sci Adv 3:e1603208
Zdanowicz, Rafal; Kreutzberger, Alex; Liang, Binyong et al. (2017) Complexin Binding to Membranes and Acceptor t-SNAREs Explains Its Clamping Effect on Fusion. Biophys J 113:1235-1250
Yang, Sung-Tae; Kreutzberger, Alex J B; Lee, Jinwoo et al. (2016) The role of cholesterol in membrane fusion. Chem Phys Lipids 199:136-143
Kreutzberger, Alex J B; Liang, Binyong; Kiessling, Volker et al. (2016) Assembly and Comparison of Plasma Membrane SNARE Acceptor Complexes. Biophys J 110:2147-50
Liang, Binyong; Tamm, Lukas K (2016) NMR as a tool to investigate the structure, dynamics and function of membrane proteins. Nat Struct Mol Biol 23:468-74
Dawidowski, Damian; Cafiso, David S (2016) Munc18-1 and the Syntaxin-1 N Terminus Regulate Open-Closed States in a t-SNARE Complex. Structure 24:392-400
Milovanovic, Dragomir; Platen, Mitja; Junius, Meike et al. (2016) Calcium Promotes the Formation of Syntaxin 1 Mesoscale Domains through Phosphatidylinositol 4,5-Bisphosphate. J Biol Chem 291:7868-76

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