Projects 1, 2 and 3 will require the use of NMR and EPR methodologies provided by Core B to investigate the structures of SNAREs and synaptotagmin and their interactions in micellar and membrane environments.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
5P01GM072694-08
Application #
8507746
Study Section
Special Emphasis Panel (ZRG1-BCMB-S)
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
8
Fiscal Year
2013
Total Cost
$88,340
Indirect Cost
$30,976
Name
University of Virginia
Department
Type
DUNS #
065391526
City
Charlottesville
State
VA
Country
United States
Zip Code
22904
Moissoglu, Konstadinos; Kiessling, Volker; Wan, Chen et al. (2014) Regulation of Rac1 translocation and activation by membrane domains and their boundaries. J Cell Sci 127:2565-76
Park, Yongsoo; Vennekate, Wensi; Yavuz, Halenur et al. (2014) ?-SNAP interferes with the zippering of the SNARE protein membrane fusion machinery. J Biol Chem 289:16326-35
Liang, Binyong; Dawidowski, Damian; Ellena, Jeffrey F et al. (2014) The SNARE motif of synaptobrevin exhibits an aqueous-interfacial partitioning that is modulated by membrane curvature. Biochemistry 53:1485-94
Hernandez, Javier M; Kreutzberger, Alex J B; Kiessling, Volker et al. (2014) Variable cooperativity in SNARE-mediated membrane fusion. Proc Natl Acad Sci U S A 111:12037-42
Cafiso, David S (2014) Identifying and quantitating conformational exchange in membrane proteins using site-directed spin labeling. Acc Chem Res 47:3102-9
Dawidowski, Damian; Cafiso, David S (2013) Allosteric control of syntaxin 1a by Munc18-1: characterization of the open and closed conformations of syntaxin. Biophys J 104:1585-94
Kiessling, Volker; Ahmed, Saheeb; Domanska, Marta K et al. (2013) Rapid fusion of synaptic vesicles with reconstituted target SNARE membranes. Biophys J 104:1950-8
Honigmann, Alf; van den Bogaart, Geert; Iraheta, Emilio et al. (2013) Phosphatidylinositol 4,5-bisphosphate clusters act as molecular beacons for vesicle recruitment. Nat Struct Mol Biol 20:679-86
van den Bogaart, Geert; Meyenberg, Karsten; Diederichsen, Ulf et al. (2012) Phosphatidylinositol 4,5-bisphosphate increases Ca2+ affinity of synaptotagmin-1 by 40-fold. J Biol Chem 287:16447-53
van den Bogaart, Geert; Thutupalli, Shashi; Risselada, Jelger H et al. (2011) Synaptotagmin-1 may be a distance regulator acting upstream of SNARE nucleation. Nat Struct Mol Biol 18:805-12

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