Projects 1, 2 and 3 will require the use of NMR and EPR methodologies provided by Core B to investigate the structures of SNAREs and synaptotagmin and their interactions in micellar and membrane environments.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
5P01GM072694-08
Application #
8507746
Study Section
Special Emphasis Panel (ZRG1-BCMB-S)
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
8
Fiscal Year
2013
Total Cost
$88,340
Indirect Cost
$30,976
Name
University of Virginia
Department
Type
DUNS #
065391526
City
Charlottesville
State
VA
Country
United States
Zip Code
22904
Yang, Sung-Tae; Lim, Sung In; Kiessling, Volker et al. (2016) Site-specific fluorescent labeling to visualize membrane translocation of a myristoyl switch protein. Sci Rep 6:32866
Dawidowski, Damian; Cafiso, David S (2016) Munc18-1 and the Syntaxin-1 N Terminus Regulate Open-Closed States in a t-SNARE Complex. Structure 24:392-400
Milovanovic, Dragomir; Platen, Mitja; Junius, Meike et al. (2016) Calcium Promotes the Formation of Syntaxin 1 Mesoscale Domains through Phosphatidylinositol 4,5-Bisphosphate. J Biol Chem 291:7868-76
Kreutzberger, Alex J B; Liang, Binyong; Kiessling, Volker et al. (2016) Assembly and Comparison of Plasma Membrane SNARE Acceptor Complexes. Biophys J 110:2147-50
Yang, Sung-Tae; Kreutzberger, Alex J B; Lee, Jinwoo et al. (2016) The role of cholesterol in membrane fusion. Chem Phys Lipids 199:136-43
Park, Yongsoo; Seo, Jong Bae; Fraind, Alicia et al. (2015) Synaptotagmin-1 binds to PIP(2)-containing membrane but not to SNAREs at physiological ionic strength. Nat Struct Mol Biol 22:815-23
Kiessling, Volker; Liang, Binyong; Tamm, Lukas K (2015) Reconstituting SNARE-mediated membrane fusion at the single liposome level. Methods Cell Biol 128:339-63
Kiessling, Volker; Yang, Sung-Tae; Tamm, Lukas K (2015) Supported lipid bilayers as models for studying membrane domains. Curr Top Membr 75:1-23
Kreutzberger, Alex J B; Kiessling, Volker; Tamm, Lukas K (2015) High cholesterol obviates a prolonged hemifusion intermediate in fast SNARE-mediated membrane fusion. Biophys J 109:319-29
Milovanovic, Dragomir; Honigmann, Alf; Koike, Seiichi et al. (2015) Hydrophobic mismatch sorts SNARE proteins into distinct membrane domains. Nat Commun 6:5984

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