Projects 1 and 2 will require high-end optical microscopy with single molecule tracking and single vesicle fusion and FRET capabilities to achieve aims 1 and 2, and 1 and 3, respectively. Project 3 will measure interbilayer distances by FLIC microscopy (aim 1) and determine synaptotagmin-induced changes in lipid order by ATR-FTIR spectroscopy (aim 3). Core A will provide the necessary state-of-the-art instrumentation, technical know-how, and personnel training to realize these aims of all three Projects. In addition, the Core will train users, provide customable software tools, and assist with image and data analysis.

Agency
National Institute of Health (NIH)
Type
Research Program Projects (P01)
Project #
5P01GM072694-09
Application #
8706173
Study Section
Special Emphasis Panel (ZRG1)
Project Start
Project End
Budget Start
Budget End
Support Year
9
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Virginia
Department
Type
DUNS #
City
Charlottesville
State
VA
Country
United States
Zip Code
22904
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