Core B provides support for the use by Program Project scientists of neutron scattering instruments at the NIST Center for Neutron Research (NCNR) in Gaithersburg, Maryland. The success of the Program Project, entitled Making Sense of Voltage Sensors, depends almost entirely on the use of neutron diffraction and reflectivity measurements to determine the disposition of potassium channels and their voltage sensors in lipid bilayer membranes.
The specific aim of Core B is to provide technical and training support at the NCNR for Program scientists using neutron scattering instruments, particularly the Advanced Neutron Diffractometer/Reflectometer (AND/R). Technical and training support will be provided by Dr. David Worcester, who is one of the early pioneers of membrane neutron diffraction. Serving as the senior neutron scientist, he will be stationed full-time at the NCNR to work closely with Project scientists to help them plan, execute, and analyze neutron scattering experiments. He will report to and work closely with the Program Director to assure the timely planning and execution of the neutron scattering work proposed in Projects 2 and 3.

Public Health Relevance

Core B is the neutron scattering core for a Program Project that investigates how ion channels gate the flow of ions across nerve, muscle, and cardiac cells in response to changes in voltage across their cell membranes. These voltage changes, called action potentials, are the means by which nerve, muscle, and cardiac cells communicate with each other. Many neuromuscular and cardiac diseases arise from defects in the way action potentials are produced. Results from this Program will help us understand the origin of such diseases.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
5P01GM086685-05
Application #
8435420
Study Section
Special Emphasis Panel (ZRG1-BCMB-N)
Project Start
Project End
2015-01-31
Budget Start
2013-02-01
Budget End
2014-01-31
Support Year
5
Fiscal Year
2013
Total Cost
$213,599
Indirect Cost
$57,376
Name
University of California Irvine
Department
Type
DUNS #
046705849
City
Irvine
State
CA
Country
United States
Zip Code
92697
Capponi, Sara; Freites, J Alfredo; Tobias, Douglas J et al. (2016) Interleaflet mixing and coupling in liquid-disordered phospholipid bilayers. Biochim Biophys Acta 1858:354-62
Amcheslavsky, Anna; Wood, Mona L; Yeromin, Andriy V et al. (2015) Molecular biophysics of Orai store-operated Ca2+ channels. Biophys J 108:237-46
Cymer, Florian; von Heijne, Gunnar; White, Stephen H (2015) Mechanisms of integral membrane protein insertion and folding. J Mol Biol 427:999-1022
Lorch, Sebastian; Capponi, Sara; Pieront, Florian et al. (2015) Dynamic Carboxylate/Water Networks on the Surface of the PsbO Subunit of Photosystem II. J Phys Chem B 119:12172-81
Worcester, David L; Weinrich, Michael (2015) Hydrostatic Pressure Promotes Domain Formation in Model Lipid Raft Membranes. J Phys Chem Lett 6:4417-21
Blasic, Joseph R; Worcester, David L; Gawrisch, Klaus et al. (2015) Pore hydration states of KcsA potassium channels in membranes. J Biol Chem 290:26765-75
Capponi, Sara; Heyden, Matthias; Bondar, Ana-Nicoleta et al. (2015) Anomalous behavior of water inside the SecY translocon. Proc Natl Acad Sci U S A 112:9016-21
Freites, J Alfredo; Tobias, Douglas J (2015) Voltage Sensing in Membranes: From Macroscopic Currents to Molecular Motions. J Membr Biol 248:419-30
Tronin, Andrey Y; Nordgren, C Erik; Strzalka, Joseph W et al. (2014) Direct evidence of conformational changes associated with voltage gating in a voltage sensor protein by time-resolved X-ray/neutron interferometry. Langmuir 30:4784-96
Jiang, Xiaoxu; Villafuerte, Maria Katerina R; Andersson, Magnus et al. (2014) Galactoside-binding site in LacY. Biochemistry 53:1536-43

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