The Microarray Core Facility will provide microarray services, and bioinformatic services, to the members of the Program Project. The microarray services will include home-made gene expression microarrays (although we are phasing this out);catalogue and custom Agilent microarrays;and catalogue Affymetric microarrays, for both gene expression and ChlP-Chip analysis. Typically the Microarray Core Facility will receive a pellet of frozen cells from the investigator. The frozen pellet will be processed to yield RNA, and this RNA will be labeled and hybridized to a micorarray. Data will be processed and loaded to an SQL database. Investigators will access this partially processed data in the database. Thus the Core facility can handle all steps from the frozen cell pellet to the storage of partially processed data. All members of the Program Project will use this facility heavily, and the commonality of methods and analysis, and the presence of all relevant data in the same database, will greatly increase our ability to interpret the data.
Understanding an alternative program for achieving a cell cycle gives us a broader view - a view with parallax - for understanding both vegetative and meiotic cell cycles, and for developmental gene expression programs in general. Furthermore, in general, the meiotic cell cycle and germ cell regulatory processes are relatively pooriy understood, though they are central to reproduction.
|Ucisik-Akkaya, Esma; Leatherwood, Janet K; Neiman, Aaron M (2014) A genome-wide screen for sporulation-defective mutants in Schizosaccharomyces pombe. G3 (Bethesda) 4:1173-82|
|Berchowitz, Luke E; Gajadhar, Aaron S; van Werven, Folkert J et al. (2013) A developmentally regulated translational control pathway establishes the meiotic chromosome segregation pattern. Genes Dev 27:2147-63|
|Lo, Hsiao-Chi; Kunz, Ryan C; Chen, Xiangyu et al. (2012) Cdc7-Dbf4 is a gene-specific regulator of meiotic transcription in yeast. Mol Cell Biol 32:541-57|
|Yuan, Hua; Rossetto, Dorine; Mellert, Hestia et al. (2012) MYST protein acetyltransferase activity requires active site lysine autoacetylation. EMBO J 31:58-70|
|Yu, Yao; Srinivasan, Madhusudhan; Nakanishi, Shima et al. (2011) A conserved patch near the C terminus of histone H4 is required for genome stability in budding yeast. Mol Cell Biol 31:2311-25|
|Neiman, Aaron M (2011) Sporulation in the budding yeast Saccharomyces cerevisiae. Genetics 189:737-65|
|Chen, Huei-Mei; Neiman, Aaron M (2011) A conserved regulatory role for antisense RNA in meiotic gene expression in yeast. Curr Opin Microbiol 14:655-9|
|Srinivasan, Madhusudhan; Mehta, Preeti; Yu, Yao et al. (2011) The highly conserved KEOPS/EKC complex is essential for a universal tRNA modification, t6A. EMBO J 30:873-81|
|Yu, Yao; Neiman, Aaron M; Sternglanz, Rolf (2010) The JmjC domain of Gis1 is dispensable for transcriptional activation. FEMS Yeast Res 10:793-801|