A robust themnosensory response in natural environments requires an animal to integrate circuits for temperature detection with circuits for other sensory modalities. The circuits for all sensory modalities must also work robustly over a range of temperatures. C elegans offers an excellent opportunity to prot)e the molecular and cellular mechanisms that regulate circuit function across different temperatures as well as mechanisms that integrate thermosensory and non-thermosensory inputs. The use of novel quantitative behavioral assays, optical neurophysiology and the rich neurogenetics available in C elegans allows us to identify and characterize the molecular, neuronal and circuit determinants of thermal robustness and multisensory integration during navigational behavior. Here, we propose to probe the interplay between thermosensory and chemosensory navigational behaviors in C elegans. The principal thermosensory neurons (AFD), NaCI-sensing neurons (ASE), and olfactory neurons for chemoattractants (AWC) are presynaptic to the same interneurons (AIY). Thus, mechanisms for multisensory integration between thermosensory and chemosensory inputs may be located in the first synapse, and moreover, the thermosensory circuit, may play a direct role in temperature-dependent regulation of the chemosensory circuit. Our proposed multifaceted analyses will uncover novel mechanisms of thermal robustness and multisensory integration, principles of which are likely to be highly conserved.

Public Health Relevance

Temperature modulates behavior and physiology in all animals. Therefore, robust sensory responses to thermal cues and to all sensory modalities at different temperatures are of critical for survival. C elegans provides us a model system to understand temperature sensing and compensation at molecular, cellular and circuit levels. These insights will help us to understand the pathology of several common human diseases, such as asthma and chronic pain, and to develop treatment of these disorders.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
5P01GM103770-02
Application #
8662281
Study Section
Special Emphasis Panel (ZRG1-CB-P)
Project Start
Project End
Budget Start
2014-05-01
Budget End
2015-04-30
Support Year
2
Fiscal Year
2014
Total Cost
$573,432
Indirect Cost
$54,987
Name
Brandeis University
Department
Type
DUNS #
616845814
City
Waltham
State
MA
Country
United States
Zip Code
02454
Goodman, Miriam B; Sengupta, Piali (2017) The extraordinary AFD thermosensor of C. elegans. Pflugers Arch :
Knecht, Zachary A; Silbering, Ana F; Cruz, Joyner et al. (2017) Ionotropic Receptor-dependent moist and dry cells control hygrosensation in Drosophila. Elife 6:
Narayan, Anusha; Venkatachalam, Vivek; Durak, Omer et al. (2016) Contrasting responses within a single neuron class enable sex-specific attraction in Caenorhabditis elegans. Proc Natl Acad Sci U S A 113:E1392-401
Lim, Maria A; Chitturi, Jyothsna; Laskova, Valeriya et al. (2016) Neuroendocrine modulation sustains the C. elegans forward motor state. Elife 5:
Berck, Matthew E; Khandelwal, Avinash; Claus, Lindsey et al. (2016) The wiring diagram of a glomerular olfactory system. Elife 5:
Ni, Lina; Klein, Mason; Svec, Kathryn V et al. (2016) The Ionotropic Receptors IR21a and IR25a mediate cool sensing in Drosophila. Elife 5:
Knecht, Zachary A; Silbering, Ana F; Ni, Lina et al. (2016) Distinct combinations of variant ionotropic glutamate receptors mediate thermosensation and hygrosensation in Drosophila. Elife 5:
Shen, Yu; Wen, Quan; Liu, He et al. (2016) An extrasynaptic GABAergic signal modulates a pattern of forward movement in Caenorhabditis elegans. Elife 5:
Venkatachalam, Vivek; Ji, Ni; Wang, Xian et al. (2016) Pan-neuronal imaging in roaming Caenorhabditis elegans. Proc Natl Acad Sci U S A 113:E1082-8
Takeishi, Asuka; Yu, Yanxun V; Hapiak, Vera M et al. (2016) Receptor-type Guanylyl Cyclases Confer Thermosensory Responses in C. elegans. Neuron 90:235-44

Showing the most recent 10 out of 19 publications