(CORE B) The Stem Cell and Trophoblast Analysis Core Unit will facilitate all aspects of research with mouse and human embryonic stem (ES) cells and rodent trophoblast stem (TS) cells. The Stem Cell and Trophoblast Analysis Core Unit will enhance the experimental capabilities of the research projects. Each of the research projects proposes to use stem cells in their research. The Core will contribute to the research effort through the following activities: i) acquisition and preparation of cell culture medium and related reagents;ii) maintaining stem cell stocks and standardization of cell culture protocols;iii) mycoplasma testing of cell cultures;iv) cell manipulation and harvesting for large-scale experimentation;v) oversee and schedule use of shared equipment;vi) coordination of genome-wide sequencing and bioinformatic analyses of stem cells;vii) development of new strategies for studying trophoblast lineage development. Access to the Stem Cell and Trophoblast Analysis Core Unit will be the most cost effective method to carry out essential aspects of the proposed experimentation. Standardizing reagents, procedures, collection of samples, and operation of essential instrumentation will significantly improve the reproducibility of the experiments. This will generate a tremendous savings in reagent costs and manpower hours. Such practices will also greatly improve experimental design, troubleshooting experiments, and interpretation of results.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Program Projects (P01)
Project #
1P01HD079363-01A1
Application #
8743036
Study Section
Special Emphasis Panel (ZHD1)
Project Start
Project End
Budget Start
2014-07-24
Budget End
2015-06-30
Support Year
1
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Kansas
Department
Type
DUNS #
City
Kansas City
State
KS
Country
United States
Zip Code
66160
Kumar, Ram P; Ray, Soma; Home, Pratik et al. (2018) Regulation of energy metabolism during early mammalian development: TEAD4 controls mitochondrial transcription. Development 145:
Soares, Michael J; Varberg, Kaela M; Iqbal, Khursheed (2018) Hemochorial placentation: development, function, and adaptations. Biol Reprod 99:196-211
Home, Pratik; Kumar, Ram Parikshan; Ganguly, Avishek et al. (2017) Genetic redundancy of GATA factors in the extraembryonic trophoblast lineage ensures the progression of preimplantation and postimplantation mammalian development. Development 144:876-888
Chakraborty, Damayanti; Muto, Masanaga; Soares, Michael J (2017) Ex vivo Trophoblast-specific Genetic Manipulation Using Lentiviral Delivery. Bio Protoc 7:
Aplin, John D; Beristain, Alexander; DaSilva-Arnold, Sonia et al. (2017) IFPA meeting 2016 workshop report III: Decidua-trophoblast interactions; trophoblast implantation and invasion; immunology at the maternal-fetal interface; placental inflammation. Placenta 60 Suppl 1:S15-S19
Dhakal, Pramod; Soares, Michael J (2017) Single-step PCR-based genetic sex determination of rat tissues and cells. Biotechniques 62:232-233
Soares, Michael J; Iqbal, Khursheed; Kozai, Keisuke (2017) Hypoxia and Placental Development. Birth Defects Res 109:1309-1329
Renaud, Stephen J; Scott, Regan L; Chakraborty, Damayanti et al. (2017) Natural killer-cell deficiency alters placental development in rats. Biol Reprod 96:145-158
Soares, Michael J; Vivian, Jay L (2016) Tipping the balance toward trophoblast development. Proc Natl Acad Sci U S A 113:5144-6
Bu, Pengli; Alam, Sheikh M Khorshed; Dhakal, Pramod et al. (2016) A Prolactin Family Paralog Regulates Placental Adaptations to a Physiological Stressor. Biol Reprod 94:107

Showing the most recent 10 out of 21 publications