Technology Implementation This proposal is a renewal application for the Stanford Genome Technology Center Grant. The promise of Whole Genome Sequencing (WGS) is now realized, and SGTC has pioneered many of the technological breakthroughs in sequence automation and high-throughput oligonucleotide synthesis that have made this a reality. Two big challenges in translational genomic medicine remain. The first is that the human organism is not a single genome, but a collection of cells with slightly varying genomes, and those variations are often very important in disease. The second is that not all regions of the genome are equivalent in disease causing potential or equally amenable to sequencing. In this third tier of the proposal. Technology Implementation, we will focus on making technologies that we have developed at SGTC accessible to a wider community of biomedical researchers and clinicians. We have developed several new technologies that are able to selectively capture highly multiplex, targeted regions ofthe genome in very small cell populations and in pools, and sequence them with very high fidelity. Successful implementation requires that the technology be: 1) robust and reproducible with low barriers for practical implementation in translational and clinical studies;and 2) low cost, such that clinical studies of large populations are done economically. We will apply targeted resequencing methods to identify rare genetic variants in cancer and provide accurate HLA typing for transplantation, and a transcriptome microarray method tailored for the cost-effective analysis of clinical samples to investigate gene expression changes. The resulting discoveries from these efforts will improve the quality of patient care by enabling biomedical researchers and clinicians to personalize treatments to each individual.

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Research Program Projects (P01)
Project #
5P01HG000205-25
Application #
8738704
Study Section
Special Emphasis Panel (ZHG1-HGR-N)
Project Start
Project End
Budget Start
2014-08-01
Budget End
2015-07-31
Support Year
25
Fiscal Year
2014
Total Cost
$1,507,953
Indirect Cost
$526,072
Name
Stanford University
Department
Type
DUNS #
009214214
City
Stanford
State
CA
Country
United States
Zip Code
94305
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