OF SERVICES Image acquisition, image processing, image analysis, and detecfion and interpretafion of fiuorescence signals are fundamental tools used in all of the projects. The availability of hardware and software to accomplish these tasks, and the availability of technical support to maintain and improve exisfing capabilifies and to develop new processing methodologies is essential for the effective and fimely completion of the proposed studies. The core includes access to confocal microscopy, flow cytometry, atomic force microscopy and total internal refiectance fluorescence microscopy (TIRFM), as well as support for the maintenance of hardware to be used for video image processing and the development of software to facilitate extraction of information from experimental images. In addifion, in the current application we propose to expand core capabilifies to include support for computational resources. Two Co-lnvestigators have been added to the project, Micah Dembo from Boston University, will provide expertise in analyzing traction force microscopy experiments, and David Gee, an Assistant Professor at Rochester Institute of Technology who holds an adjunct appointment in Biomedical Engineering at the University of Rochester, who is expert in parallel compufing applications. Thus, capabilities for both high end parallel compufing resources and the ability to analyze traction force microscopy experiments represent significant addifions to services provided by the Core. Projects 1, 2, 3 and 5 are engaged in research directions that will benefit from these new resources, and Project 4, to a lesser extent, will benefit from the availability of these capabilities. We have made progress in the past grant period in moving to new hardware platforms, moving to a largely digital imaging acquisition format. One of the primary objectives for the Core in the next funding period will be to provide the technical support and resources for improving capabilifies of this new hardware and facilitafing its roufine use in the proposed studies. We also recognize that the digital worid is constantly evolving, and confinued support to troubleshoot established technologies and maintain needed levels of imaging capability will be needed throughout the funded period of the program.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL018208-36
Application #
8377776
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2012-07-01
Budget End
2013-06-30
Support Year
36
Fiscal Year
2012
Total Cost
$182,928
Indirect Cost
$34,118
Name
University of Rochester
Department
Type
DUNS #
041294109
City
Rochester
State
NY
Country
United States
Zip Code
14627
Vats, Kanika; Marsh, Graham; Harding, Kristen et al. (2017) Nanoscale physicochemical properties of chain- and step-growth polymerized PEG hydrogels affect cell-material interactions. J Biomed Mater Res A 105:1112-1122
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Rocheleau, Anne D; Wang, Weiwei; King, Michael R (2016) Effect of Pseudopod Extensions on Neutrophil Hemodynamic Transport Near a Wall. Cell Mol Bioeng 9:85-95
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MacKay, Joanna L; Hammer, Daniel A (2016) Stiff substrates enhance monocytic cell capture through E-selectin but not P-selectin. Integr Biol (Camb) 8:62-72
Hind, Laurel E; Lurier, Emily B; Dembo, Micah et al. (2016) Effect of M1-M2 Polarization on the Motility and Traction Stresses of Primary Human Macrophages. Cell Mol Bioeng 9:455-465
Hughes, Andrew D; Marsh, Graham; Waugh, Richard E et al. (2015) Halloysite Nanotube Coatings Suppress Leukocyte Spreading. Langmuir 31:13553-60
Lim, Kihong; Hyun, Young-Min; Lambert-Emo, Kris et al. (2015) Visualization of integrin Mac-1 in vivo. J Immunol Methods 426:120-7

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