Atherosclerosis and Lipoprotein Analysis Core (Core C). Jonathan Smith, Ph.D., Core Leader Project Summary/Abstract The Lipoprotein analysis portion of this Core's primary objective is to analyze lipid and lipoprotein levels in various mouse models produced by projects and to provide human lipoproteins as needed. Mouse lipoprotein analysis will be accomplished using two methods, one based on separation of HDL-C from non-HDL-C via ultracentrifugation or precipitation, and the other based on size exclusion chromatography by FPLC. The Atherosclerosis portion of this Core's primary objective is to train investigators how to sacrifice mice and prepare tissues for atherosclerosis assays, and to offer two separate quantitative atherosclerosis assays; 1) cross sectional lesion area at the aortic root, and 2) cross sectional brachiocephalic lesion area. We can also assist Project personnel by teaching them the assay of surface lesion area on en face preparations of the entire aorta. Standardization of the lipoprotein assays and atherosclerosis assays will facilitate inter-laboratory collaboration and data comparisons. In addition, the Core will relieve the individual laboratories from having to learn and perform the laborious assays for atherosclerosis quantification.

Public Health Relevance

Atherosclerosis and Lipoprotein Analysis Core (Core C). Project Narrative Core C will provide services to all three projects to increase productivity. These services include providing lipoproteins, the determination of lipoprotein cholesterol and triglyceride levels by buoyant density and size (FPLC) fractionation, and the determination of mouse atherosclerotic lesion areas in the aortic root and brachiocephalic artery.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL029582-35
Application #
9607625
Study Section
Special Emphasis Panel (ZHL1)
Program Officer
Hasan, Ahmed a K
Project Start
Project End
Budget Start
2018-11-01
Budget End
2019-10-31
Support Year
35
Fiscal Year
2019
Total Cost
Indirect Cost
Name
Cleveland Clinic Lerner
Department
Type
DUNS #
135781701
City
Cleveland
State
OH
Country
United States
Zip Code
44195
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