The CYP-derived eicosanoids including EETs and 20-HETE are at the center of the proposed research in each project of this Program Project. Accordingly, a centralized core facility to measure their levels in tissues and biological fluids is critical for the execution and successful completion of the proposed studies. Mass spectrometry remains the state-of-the art methodology for quantitative analysis of eicosanoids and their derivatives as well as for structural identification of new lipid molecules. The centralized Mass Spectroscopy Core will continue to provide investigators in this program with a consolidated, highly specialized, well equipped and professionally staffed unit capable of performing a variety of mass spectroscopy-based analyses of various eicosanoids, including advanced liquid chromatography-tandem mass spectrometry (LC/MS/MS) and gas chromatography-mass spectrometry (GC/MS). This centralized core facility ensures uniformity of assay results between the different projects while maintaining the highest quality of analytical precision and accuracy achievable;it will keep complete, computerized and centralized records as well as well-defined quality control guidelines for each method. Consequently, the specific objectives of the Core are: 1) To establish and ensure uniform standardized methods for preparing samples for analyses including sample collection, extraction and storage till analyses;2) To provide reliable, reproducible and timely mass spectroscopy analyses of CYP-derived eicosanoids in tissues and biological fluids (plasma, urine, etc.);3) To maintain computerized records of raw data and analyzed results;4)To assist investigators in developing new methods. Utilization of mass spectrometry at many levels is an important feature ofthis Program Project proposal. This stems from the recognition that mass spectrometric analyses provide adequate sensitivity, specificity, and selectivity required for detection and characterization of subpicomolar quantities of eicosanoids. No other technique of comparable sensitivity is currently available for their characterization and quantification. All Program Project Investigators will utilize the mass spectrometry for one or more aspects of quantitative, structural and chiral analyses.

Public Health Relevance

(See inslructions): The ability to identify, detect and quantify CYP-eicosanoids and related metabolites in tissues, cells and biological fluids is vital to the success of this Program Project. This Mass Spectrometry Core has the physical and intellectual resources needed to support Program Project Investigators in elucidating the role and function of these eicosanoids in health and disease.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Program Projects (P01)
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Heart, Lung, and Blood Initial Review Group (HLBP)
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New York Medical College
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Wang, Lijun; Zhang, Chengbiao; Su, Xiao-Tong et al. (2017) PGF2? regulates the basolateral K channels in the distal convoluted tubule. Am J Physiol Renal Physiol 313:F254-F261
Garcia, Victor; Gilani, Ankit; Shkolnik, Brian et al. (2017) 20-HETE Signals Through G-Protein-Coupled Receptor GPR75 (Gq) to Affect Vascular Function and Trigger Hypertension. Circ Res 120:1776-1788
Bowden, John A; Heckert, Alan; Ulmer, Candice Z et al. (2017) Harmonizing lipidomics: NIST interlaboratory comparison exercise for lipidomics using SRM 1950-Metabolites in Frozen Human Plasma. J Lipid Res 58:2275-2288
Zhang, Hui; Falck, John R; Roman, Richard J et al. (2017) Upregulation of 20-HETE Synthetic Cytochrome P450 Isoforms by Oxygen-Glucose Deprivation in Cortical Neurons. Cell Mol Neurobiol 37:1279-1286
Pandey, Varunkumar; Garcia, Victor; Gilani, Ankit et al. (2017) The Blood Pressure-Lowering Effect of 20-HETE Blockade in Cyp4a14(-/-) Mice Is Associated with Natriuresis. J Pharmacol Exp Ther 363:412-418
Sodhi, Komal; Srikanthan, Krithika; Goguet-Rubio, Perrine et al. (2017) pNaKtide Attenuates Steatohepatitis and Atherosclerosis by Blocking Na/K-ATPase/ROS Amplification in C57Bl6 and ApoE Knockout Mice Fed a Western Diet. Sci Rep 7:193
Chen, Li; Joseph, Gregory; Zhang, Frank F et al. (2016) 20-HETE contributes to ischemia-induced angiogenesis. Vascul Pharmacol 83:57-65
Qin, Jun; Le, Yicong; Froogh, Ghezal et al. (2016) Sexually dimorphic adaptation of cardiac function: roles of epoxyeicosatrienoic acid and peroxisome proliferator-activated receptors. Physiol Rep 4:
Singh, Shailendra P; Schragenheim, Joseph; Cao, Jian et al. (2016) PGC-1 alpha regulates HO-1 expression, mitochondrial dynamics and biogenesis: Role of epoxyeicosatrienoic acid. Prostaglandins Other Lipid Mediat 125:8-18
Su, Xiao-Tong; Zhang, Chengbiao; Wang, Lijun et al. (2016) Disruption of KCNJ10 (Kir4.1) stimulates the expression of ENaC in the collecting duct. Am J Physiol Renal Physiol 310:F985-93

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