This CELL CORE has continuously served this Program Project for 25 years and has all necessary infrastructure, equipment, space and personnel. Dr. Henson originated the human blood cell isolation procedures that are still in use for the provision of leukocytes. Dr. Bratton has overseen the blood cell isolation and regulatory management of this resource for many years now. Dr. Leslie and her laboratory have provided consistent, continuous macrophage isolation and cell culture. Human alveolar macrophages provided by our collaborator Dr. Robert Mason (see letter) to the CELL CORE will be cultured and distributed to the Projects. Sources of the provided cells are four: 1) isolated human blood cells, 2) human alveolar macrophages, 3) resident macrophages from the lungs and peritonea and bone marrow cells for macrophage derivation from knockout and wild type mice, and 4) cultured cell lines. Brief methods for cell isolation and culture are described and a reference list detailing procedures to be utilized is included. The description of dedicated space, equipment and necessary resources is outlined in the Resources and Environment section. The Budget Justification for the CELL CORE reflects services and resources to be utilized in common for two or more Projects.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL034303-28
Application #
8426128
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2013-02-01
Budget End
2014-01-31
Support Year
28
Fiscal Year
2013
Total Cost
$275,495
Indirect Cost
$66,515
Name
National Jewish Health
Department
Type
DUNS #
076443019
City
Denver
State
CO
Country
United States
Zip Code
80206
Zemski Berry, Karin A; Murphy, Robert C; Kosmider, Beata et al. (2017) Lipidomic characterization and localization of phospholipids in the human lung. J Lipid Res 58:926-933
Mould, Kara J; Barthel, Lea; Mohning, Michael P et al. (2017) Cell Origin Dictates Programming of Resident versus Recruited Macrophages during Acute Lung Injury. Am J Respir Cell Mol Biol 57:294-306
Gibbings, Sophie L; Thomas, Stacey M; Atif, Shaikh M et al. (2017) Three Unique Interstitial Macrophages in the Murine Lung at Steady State. Am J Respir Cell Mol Biol 57:66-76
Yun, Bogeon; Lee, HeeJung; Jayaraja, Sabarirajan et al. (2016) Prostaglandins from Cytosolic Phospholipase A2?/Cyclooxygenase-1 Pathway and Mitogen-activated Protein Kinases Regulate Gene Expression in Candida albicans-infected Macrophages. J Biol Chem 291:7070-86
Desch, A Nicole; Gibbings, Sophie L; Goyal, Rajni et al. (2016) Flow Cytometric Analysis of Mononuclear Phagocytes in Nondiseased Human Lung and Lung-Draining Lymph Nodes. Am J Respir Crit Care Med 193:614-26
Jayaraja, Sabarirajan; Dakhama, Azzeddine; Yun, Bogeon et al. (2016) Cytosolic phospholipase A2 contributes to innate immune defense against Candida albicans lung infection. BMC Immunol 17:27
Kandasamy, Pitchaimani; Numata, Mari; Berry, Karin Zemski et al. (2016) Structural analogs of pulmonary surfactant phosphatidylglycerol inhibit toll-like receptor 2 and 4 signaling. J Lipid Res 57:993-1005
Zemski Berry, Karin A; Murphy, Robert C (2016) Phospholipid Ozonation Products Activate the 5-Lipoxygenase Pathway in Macrophages. Chem Res Toxicol 29:1355-64
Frasch, S Courtney; McNamee, Eóin N; Kominsky, Douglas et al. (2016) G2A Signaling Dampens Colitic Inflammation via Production of IFN-?. J Immunol 197:1425-34
Janssen, William J; Bratton, Donna L; Jakubzick, Claudia V et al. (2016) Myeloid Cell Turnover and Clearance. Microbiol Spectr 4:

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