The Core is structured so that all the work is performed by Core personnel. Individual investigators who wish to use cells or other materials or to develop new models discuss the type of cells needed, the culture conditions, and the overall goal of the experiment or project with Core personnel. Then, Core personnel provide the cells and models to the investigator. Frequently, the individual investigator and Core personnel work together to provide the optimal conditions (media, seeding density, duration of incubation, substrates, passage number, etc) required for a specific type of study. The development of new models is usually an iterative process, with investigators talking to the Core, Core personnel developing a model, the investigator evaluating it, and making suggestions, the Core personnel attempting to improve the model, and so on. The organization of the Core has several advantages, a) It provides the highest level of quality control. We rarely have infected cell lines and alterations in phenotype are detected eariy. b) It provides the best use of facilities, c) By having Core personnel do all the work of the Core, traffic and time conflicts are minimized, d) It provides PPG investigators with a highly experienced and innovative staff who are committed to developing models that will allow investigators to generate new insight into CF. The Core is located in approximately 550 square feet of space in rooms 567, 571, and 500B of the Eckstein Medical Research Building (EMRB). EMRB contains the laboratories of all 4 project Pis (Drs. Davidson, Welsh, McCray, and Zabner).
| Meyerholz, David K; Sieren, Jessica C; Beck, Amanda P et al. (2018) Approaches to Evaluate Lung Inflammation in Translational Research. Vet Pathol 55:42-52 |
| Rosen, Bradley H; Evans, T Idil Apak; Moll, Shashanna R et al. (2018) Infection Is Not Required for Mucoinflammatory Lung Disease in CFTR-Knockout Ferrets. Am J Respir Crit Care Med 197:1308-1318 |
| Mao, Suifang; Shah, Alok S; Moninger, Thomas O et al. (2018) Motile cilia of human airway epithelia contain hedgehog signaling components that mediate noncanonical hedgehog signaling. Proc Natl Acad Sci U S A 115:1370-1375 |
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| Meyerholz, David K; Stoltz, David A; Gansemer, Nick D et al. (2018) Lack of cystic fibrosis transmembrane conductance regulator disrupts fetal airway development in pigs. Lab Invest 98:825-838 |
| Gray, Robert D; Hardisty, Gareth; Regan, Kate H et al. (2018) Delayed neutrophil apoptosis enhances NET formation in cystic fibrosis. Thorax 73:134-144 |
| Thornell, Ian M; Li, Xiaopeng; Tang, Xiao Xiao et al. (2018) Nominal carbonic anhydrase activity minimizes airway-surface liquid pH changes during breathing. Physiol Rep 6: |
| Reznikov, Leah R; Meyerholz, David K; Abou Alaiwa, Mahmoud et al. (2018) The vagal ganglia transcriptome identifies candidate therapeutics for airway hyperreactivity. Am J Physiol Lung Cell Mol Physiol 315:L133-L148 |
| Meyerholz, David K; Beck, Amanda P; Goeken, J Adam et al. (2018) Glycogen depletion can increase the specificity of mucin detection in airway tissues. BMC Res Notes 11:763 |
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