The goal of the Pathology Core is to provide professional services and expertise to Investigators of the ?Gene Therapy for Cystic Fibrosis Lung Disease? Program Project. To facilitate these goals the Pathology Core offers 1) technical expertise and support for histotechnology techniques from tissue processing to specialized staining (histochemical and immunohistochemical) and 2) ACVP-boarded veterinary pathologist expertise and support for morphologic, quantitative and semi-quantitative evaluation of tissues (e.g. sinus and lung) from CF pig and CF ferret studies. The Pathology Core will provide services to Program Investigators in a timely and efficient manner for optimal assessment of tissues.
The Specific Aims of the Pathology Core are: 1) Provide scientific support and expertise to Program Investigators for Histology and Veterinary Pathology applications. 2) Apply quantitaive, semi-quantitative, and morphologic (e.g. cell type) techniques to assess CFTR or reporter gene expression in cell types following vector delivery (e.g. AAV, lentivirus, adenovirus ? Projects 1, 3) using CFTR or reporter (e.g. GFP, mCherry) detection methods such as immunohistochemistry and fluorescence microscopy. 3) Apply quantitative, semi-quantitative, and morphologic techniques to assess changes in disease state of sinus or lung following CFTR expression from gene transfer vectors (Projects 1, 3) or transgenic expression of CFTR in CF pigs and ferrets (Projects 2, 3).
| Meyerholz, David K; Sieren, Jessica C; Beck, Amanda P et al. (2018) Approaches to Evaluate Lung Inflammation in Translational Research. Vet Pathol 55:42-52 |
| Rosen, Bradley H; Evans, T Idil Apak; Moll, Shashanna R et al. (2018) Infection Is Not Required for Mucoinflammatory Lung Disease in CFTR-Knockout Ferrets. Am J Respir Crit Care Med 197:1308-1318 |
| Mao, Suifang; Shah, Alok S; Moninger, Thomas O et al. (2018) Motile cilia of human airway epithelia contain hedgehog signaling components that mediate noncanonical hedgehog signaling. Proc Natl Acad Sci U S A 115:1370-1375 |
| Montoro, Daniel T; Haber, Adam L; Biton, Moshe et al. (2018) A revised airway epithelial hierarchy includes CFTR-expressing ionocytes. Nature 560:319-324 |
| Lynch, Thomas J; Anderson, Preston J; Rotti, Pavana G et al. (2018) Submucosal Gland Myoepithelial Cells Are Reserve Stem Cells That Can Regenerate Mouse Tracheal Epithelium. Cell Stem Cell 22:653-667.e5 |
| Meyerholz, David K; Stoltz, David A; Gansemer, Nick D et al. (2018) Lack of cystic fibrosis transmembrane conductance regulator disrupts fetal airway development in pigs. Lab Invest 98:825-838 |
| Gray, Robert D; Hardisty, Gareth; Regan, Kate H et al. (2018) Delayed neutrophil apoptosis enhances NET formation in cystic fibrosis. Thorax 73:134-144 |
| Thornell, Ian M; Li, Xiaopeng; Tang, Xiao Xiao et al. (2018) Nominal carbonic anhydrase activity minimizes airway-surface liquid pH changes during breathing. Physiol Rep 6: |
| Reznikov, Leah R; Meyerholz, David K; Abou Alaiwa, Mahmoud et al. (2018) The vagal ganglia transcriptome identifies candidate therapeutics for airway hyperreactivity. Am J Physiol Lung Cell Mol Physiol 315:L133-L148 |
| Meyerholz, David K; Beck, Amanda P; Goeken, J Adam et al. (2018) Glycogen depletion can increase the specificity of mucin detection in airway tissues. BMC Res Notes 11:763 |
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