The protein expression core is designed to continue to provide highly purified recombinant proteins to each of the component projects. The core will provide advice about preparing cDNA expression constructs. Prokaryotic expression will be achieved primarily by use of vectors such as PET-15 or as fusion proteins with glutathione S-transferase or maltose-binding protein using vectors such as pGEX or pMAL. In selected cases, expression in insect cells will be employed to produce recombinant Fab fragments or when critical post-translational modifications are required for activity. The core will perform microbiological manipulations and fermentations. Proteins will be purified by affinity chromatography and fast protein and high performance liquid chromatography and chemically characterized by mass spectroscopy. The core will also prepare specialized novel model protein mimics of integrin cytoplasmic domains for analysis of interactions at the cytoplasmic face of these receptors

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL057900-15
Application #
8213152
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2011-02-01
Budget End
2012-01-31
Support Year
15
Fiscal Year
2011
Total Cost
$155,262
Indirect Cost
Name
University of California San Diego
Department
Type
DUNS #
804355790
City
La Jolla
State
CA
Country
United States
Zip Code
92093
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