The Core Leader, Dr. Hilenski, has demonstrated capabilities in fluorescence microscopy and immunocytochemistry of cultured cells (1-10) and immunohistochemistry of tissues (11, 12). The Microscopy and Histology Core was established in 2000, with Dr. Hilenski appointed as director. Since this time, the core facility has supported studies on (a) detection of ROS production by cytochemical fluorescence methods within arteries both in animal (13-16) and in human vascular disease (17);(b) the localization of signaling molecules in angiotensin II (5, 8, 18, 19), redox-sensitive (7) and angiogenic (7, 9, 10, 20) pathways in vascular cells and tissues;(c) localization of NADPH oxidase components in vascular cells and tissues (6, 21, 22);(d) characterization of transgenic mouse models for hypertension (23) and hypertrophy (24);(e) morphometric measurements of vascular hypertrophy (25, 26) and atherosclerotic lesion size (24, 27);(f) immunostaining of ischemic and nonischemic tissues in mouse models of impaired neovascularization (28);(g) immunohistochemical staining of cell-specific (17, 29), inflammatory (30, 31) and atherosclerotic markers in lesions and vascular wall remodeling (29, 31-33);(h) localization of fluorescent eNOS mRNA and actin isoforms in endothelial cells (34);(i) immunohistochemical staining of bone morphogenic protein 4 (BMP4) and secreted BMP antagonists in cultured bovine endothelial cells and in mouse and human blood vessels exposed to unstable flow conditions (35);and (j) immunostaining of inflammatory cells in aortas in mouse models of hypertension (26).

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
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Heart, Lung, and Blood Initial Review Group (HLBP)
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Emory University
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Kim, C; Fang, F; Weyand, C M et al. (2017) The life cycle of a T cell after vaccination - where does immune ageing strike? Clin Exp Immunol 187:71-81
Loperena, Roxana; Harrison, David G (2017) Oxidative Stress and Hypertensive Diseases. Med Clin North Am 101:169-193
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Li, Yinyin; Shen, Yi; Hohensinner, Philipp et al. (2016) Deficient Activity of the Nuclease MRE11A Induces T Cell Aging and Promotes Arthritogenic Effector Functions in Patients with Rheumatoid Arthritis. Immunity 45:903-916
Wen, Zhenke; Shimojima, Yasuhiro; Shirai, Tsuyoshi et al. (2016) NADPH oxidase deficiency underlies dysfunction of aged CD8+ Tregs. J Clin Invest 126:1953-67
Itani, Hana A; McMaster Jr, William G; Saleh, Mohamed A et al. (2016) Activation of Human T Cells in Hypertension: Studies of Humanized Mice and Hypertensive Humans. Hypertension 68:123-32
Shirai, Tsuyoshi; Nazarewicz, Rafal R; Wallis, Barbara B et al. (2016) The glycolytic enzyme PKM2 bridges metabolic and inflammatory dysfunction in coronary artery disease. J Exp Med 213:337-54

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