This Core will be housed at the Cincinnati Children's Hospital Medical Center within the Division of Molecular Cardiovascular Biology. Although spatially separated from members of the Program Project Grant team. Dr. Robbins has been collaborating with them for years and supplies the current Program Project Grant investigators and Cores with both mice and protein fragments. The Core will be critical for the studies proposed in all of the Program Project Grant's Component's as it will be responsible for the design, production, colony maintenance and shipment of ail transgenic mice that are proposed by the Program Project Grant's investigators and Core B. The Core will also produce the different proteins, protein fragments and antibodies needed for the in vitro and isolated systems studies. As such, it forms an integral part of the Program Project Grant's foundation. The Core's responsibilities are: ? Design and build all constructs for bacterial- or Sacu/o-based MyBP-C protein and protein fragment production. ? Grow and purify all protein fragments in sufficient quantities to satisfy the investigators'needs ? Design, build and generate all transgenic mice that are proposed in the Program Project Grant. ? Maintain sufficient numbers of all lines in barrier facilities so that they can be distributed as needed to the investigators as well as to other scientists who request them. ? Serve as a central clearing house for the preparation, maintenance and quality control of the cMyBP-C specific antibodies ? Maintain an adequate inventory of all of the above and arrange for convenient shipping to the individual investigators or Core Leaders.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
2P01HL059408-11
Application #
7789884
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
2010-02-01
Project End
2015-01-31
Budget Start
2010-02-01
Budget End
2011-01-31
Support Year
11
Fiscal Year
2010
Total Cost
$305,269
Indirect Cost
Name
University of Vermont & St Agric College
Department
Type
DUNS #
066811191
City
Burlington
State
VT
Country
United States
Zip Code
05405
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Previs, Michael J; Mun, Ji Young; Michalek, Arthur J et al. (2016) Phosphorylation and calcium antagonistically tune myosin-binding protein C's structure and function. Proc Natl Acad Sci U S A 113:3239-44
Bhuiyan, Md Shenuarin; McLendon, Patrick; James, Jeanne et al. (2016) In vivo definition of cardiac myosin-binding protein C's critical interactions with myosin. Pflugers Arch 468:1685-95
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James, Jeanne; Robbins, Jeffrey (2016) Healing a Heart Through Genetic Intervention. Circ Res 118:920-2

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