The overall goals of Core D are to provide (i) fiuorescence, confocal, and electron microscopy support, (ii) image analysis, and (iii) physiological support for lung perfusion experiments proposed in all projects. Centralization of the imaging and physiological support within a single core refiects the emphasis that P.l.s have placed on imaging and physiological studies in lung models. Core D is essential in order to fulfill the objectives of all projects. In addition to the research support. Core D personnel will also provide training for project participants in these methodologies. Core D will provide expertise, resources, and equipment for performing lung studies in the knockout mouse models and other mouse models in which proteins of interest are expressed through gene delivery via liposomes. Core D will provide expertise for the transfection of cDNAs in mouse lung microvessels using cationic liposomes. The physiological support component will provide standardized methods for quantification of lung vascular permeability in normal and genetically modified mice. This will include measurement of pulmonary capillary filtration coefficient and vessel wall albumin permeability surface-area product. The methods to be used have been developed specifically for the mouse lung. In addition, lung vascular albumin permeability and the routes of albumin transport will be assessed by electron microscopy and morphometric analysis using described methods. In terms of the imaging component. Core D will provide resources and expertise for (i) live cell and fixed specimen fluorescence, confocal, FRET (fluorescence resonance energy transfer), and TIRF (total internal reflective fluorescence) microscopy and (ii) transmission electron microscopy. Also, Core D will provide assessment of expression of transfected and liposome-delivered proteins by fluorescence and confocal microscopy of endothelial monolayers and whole mount lung sections as described.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL060678-14
Application #
8620697
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2014-03-01
Budget End
2015-02-28
Support Year
14
Fiscal Year
2014
Total Cost
$442,739
Indirect Cost
$160,740
Name
University of Illinois at Chicago
Department
Type
DUNS #
098987217
City
Chicago
State
IL
Country
United States
Zip Code
60612
Liu, Yuru; Kumar, Varsha Suresh; Zhang, Wei et al. (2015) Activation of type II cells into regenerative stem cell antigen-1(+) cells during alveolar repair. Am J Respir Cell Mol Biol 53:113-24
Tang, Haiyang; Chen, Jiwang; Fraidenburg, Dustin R et al. (2015) Deficiency of Akt1, but not Akt2, attenuates the development of pulmonary hypertension. Am J Physiol Lung Cell Mol Physiol 308:L208-20
Piegeler, Tobias; Dull, Randal O; Hu, Guochang et al. (2014) Ropivacaine attenuates endotoxin plus hyperinflation-mediated acute lung injury via inhibition of early-onset Src-dependent signaling. BMC Anesthesiol 14:57
Mao, Mao; Varadarajan, Sudhahar; Fukai, Tohru et al. (2014) Nitroglycerin tolerance in caveolin-1 deficient mice. PLoS One 9:e104101
Chernaya, Olga; Shinin, Vasily; Liu, Yuru et al. (2014) Behavioral heterogeneity of adult mouse lung epithelial progenitor cells. Stem Cells Dev 23:2744-57
Thangavel, Jayakumar; Malik, Asrar B; Elias, Harold K et al. (2014) Combinatorial therapy with acetylation and methylation modifiers attenuates lung vascular hyperpermeability in endotoxemia-induced mouse inflammatory lung injury. Am J Pathol 184:2237-49
Tobin, Matthew K; Bonds, Jacqueline A; Minshall, Richard D et al. (2014) Neurogenesis and inflammation after ischemic stroke: what is known and where we go from here. J Cereb Blood Flow Metab 34:1573-84
Yang, Kai-Chien; Rutledge, Cody A; Mao, Mao et al. (2014) Caveolin-1 modulates cardiac gap junction homeostasis and arrhythmogenecity by regulating cSrc tyrosine kinase. Circ Arrhythm Electrophysiol 7:701-10
Mehta, Dolly; Ravindran, Krishnan; Kuebler, Wolfgang M (2014) Novel regulators of endothelial barrier function. Am J Physiol Lung Cell Mol Physiol 307:L924-35
Mittal, Manish; Siddiqui, Mohammad Rizwan; Tran, Khiem et al. (2014) Reactive oxygen species in inflammation and tissue injury. Antioxid Redox Signal 20:1126-67

Showing the most recent 10 out of 137 publications