Core C: Mouse Phenotyping Core The Mouse Phenotyping Core will establish and make available to investigators a broad range of mouse models of cardiovascular function and dysfunction. These include (1) wire injury to the femoral or carotid arteries;(ii) low flow dependent model of the carotid which retains endothelial integrity and (iii) an abdominal aortic aneurysm formation in response to angiotensin II infusion in hyperlipidemic mice. Core personnel will train individuals from each of the Projects in the use of these models. Similarly, core personnel will harvest vascular cells and platelets from these models for DNA analysis and/or studies of function. An additional role of the core will be to develop and characterize new mouse models and phenotypic approaches prior to their export to Project laboratories. The Mouse Phenotyping Core is equipped for constant hemodynamic monitoring, urine collection in metabolic cages and blood sampling, cellular isolation and storage. Facilities exist for monitoring whole blood and platelet aggregation and studies of platelet adhesion.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Program Projects (P01)
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Heart, Lung, and Blood Initial Review Group (HLBP)
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University of Pennsylvania
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Jiang, Yi-Zhou; Jiménez, Juan M; Ou, Kristy et al. (2014) Hemodynamic disturbed flow induces differential DNA methylation of endothelial Kruppel-Like Factor 4 promoter in vitro and in vivo. Circ Res 115:32-43
Jiménez, Juan M; Prasad, Varesh; Yu, Michael D et al. (2014) Macro- and microscale variables regulate stent haemodynamics, fibrin deposition and thrombomodulin expression. J R Soc Interface 11:20131079
Tang, Soon Yew; Monslow, James; Todd, Leslie et al. (2014) Cyclooxygenase-2 in endothelial and vascular smooth muscle cells restrains atherogenesis in hyperlipidemic mice. Circulation 129:1761-9
Bae, Yong Ho; Mui, Keeley L; Hsu, Bernadette Y et al. (2014) A FAK-Cas-Rac-lamellipodin signaling module transduces extracellular matrix stiffness into mechanosensitive cell cycling. Sci Signal 7:ra57
Davies, Peter F; Manduchi, Elisabetta; Stoeckert, Christian J et al. (2014) Emerging topic: flow-related epigenetic regulation of endothelial phenotype through DNA methylation. Vascul Pharmacol 62:88-93
Chen, Lihong; Yang, Guangrui; Monslow, James et al. (2014) Myeloid cell microsomal prostaglandin E synthase-1 fosters atherogenesis in mice. Proc Natl Acad Sci U S A 111:6828-33
Yu, Zhou; Ricciotti, Emanuela; Miwa, Takashi et al. (2013) Myeloid cell 5-lipoxygenase activating protein modulates the response to vascular injury. Circ Res 112:432-40
Fitzgerald, Desmond J; Fitzgerald, Garret A (2013) Historical lessons in translational medicine: cyclooxygenase inhibition and P2Y12 antagonism. Circ Res 112:174-94
Lopes, Joshua; Adiguzel, Eser; Gu, Steven et al. (2013) Type VIII collagen mediates vessel wall remodeling after arterial injury and fibrous cap formation in atherosclerosis. Am J Pathol 182:2241-53
Castagnino, Paola; Kothapalli, Devashish; Hawthorne, Elizabeth A et al. (2013) miR-221/222 compensates for Skp2-mediated p27 degradation and is a primary target of cell cycle regulation by prostacyclin and cAMP. PLoS One 8:e56140

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