Core A, Microscopy, comprises valuable, electron and modern light microscopic tools that are made available as routine services to Program Project members. The services provided have been crucial for individual laboratory projects, and intra-programmatic research. Core A was established in the original application to meet the growing needs of the investigators in this area and has expanded in this renewal to include Dr. Farquhar as the core leader bringing her expertise as a microscopist due to the recent retirement of Dr. Feramisco. Electron microscopy facilities under her direction are located in the Center for Molecular Medicine and contain state of the art microscopes and specialized preparatory equipment. The core also includes Dr. Patel as a co-investigator who has expertise in light microscopy and has worked over ten years with Dr. Feramisco and the newly established School of Medicine (SOM) Microscopy Core which includes 2- photon confocal, spinning disk, super-resolution (purchase planned) and deconvolution systems. We propose in this application to fund two part-time research assistants to provide dedicated support to PPG members by 1) providing standardized training in the use of electron microscopy and 3-D fluorescence microscopes, 2) training individuals within this PPG to take full advantage of the enhanced scientific visualization software available in our group, and 3) providing hands-on help with the use of the many microscopes available. With increasing frequency, current PPG investigators are making use of these enhanced features, and the proposed Core A will facilitate the ability of PPG members to access these services in a timely and cost-efficient manner.

Public Health Relevance

Core A will provide basic and sophisticated tools including multiple microscopes, important training and, experimental design for PPG members. The goal will be to provide the best possible resources to visualize proteins and protein-protein interactions inside a cell specific to each project and experimental situation in a timely and cost effective manner.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Program Projects (P01)
Project #
Application #
Study Section
Heart, Lung, and Blood Program Project Review Committee (HLBP)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Veterans Medical Research Fdn/San Diego
San Diego
United States
Zip Code
Pandey, Amit K; Penny, William F; Bhargava, Valmik et al. (2016) Clinical Evaluation of Heart Failure: Agreement among Tests. PLoS One 11:e0161536
Gao, Mei Hua; Giamouridis, Dimosthenis; Lai, N Chin et al. (2016) One-time injection of AAV8 encoding urocortin 2 provides long-term resolution of insulin resistance. JCI Insight 1:e88322
Schilling, Jan M; Horikawa, Yousuke T; Zemljic-Harpf, Alice E et al. (2016) Electrophysiology and metabolism of caveolin-3-overexpressing mice. Basic Res Cardiol 111:28
Cividini, Federico; Scott, Brian T; Dai, Anzhi et al. (2016) O-GlcNAcylation of 8-Oxoguanine DNA Glycosylase (Ogg1) Impairs Oxidative Mitochondrial DNA Lesion Repair in Diabetic Hearts. J Biol Chem 291:26515-26528
See Hoe, Louise E; Schilling, Jan M; Busija, Anna R et al. (2016) Chronic β1-adrenoceptor blockade impairs ischaemic tolerance and preconditioning in murine myocardium. Eur J Pharmacol 789:1-7
Tran, Chinh; Stary, Creed M; Schilling, Jan M et al. (2015) Role of caveolin-3 in lymphocyte activation. Life Sci 121:35-9
Lai, N Chin; Gao, Mei Hua; Giamouridis, Dimosthenis et al. (2015) Intravenous AAV8 Encoding Urocortin-2 Increases Function of the Failing Heart in Mice. Hum Gene Ther 26:347-56
Schilling, Jan M; Roth, David M; Patel, Hemal H (2015) Caveolins in cardioprotection - translatability and mechanisms. Br J Pharmacol 172:2114-25
Sun, Junhui; Nguyen, Tiffany; Aponte, Angel M et al. (2015) Ischaemic preconditioning preferentially increases protein S-nitrosylation in subsarcolemmal mitochondria. Cardiovasc Res 106:227-36
Markandeya, Yogananda S; Phelan, Laura J; Woon, Marites T et al. (2015) Caveolin-3 Overexpression Attenuates Cardiac Hypertrophy via Inhibition of T-type Ca2+ Current Modulated by Protein Kinase Cα in Cardiomyocytes. J Biol Chem 290:22085-100

Showing the most recent 10 out of 75 publications