The purpose of the Vector Production Core (Core B) is to develop and provide gene transfer reagents to members of the proposed Program efficiently and in a cost-effective manner. The research-grade vector development/production laboratory has been well established and centralized core component of the UCSD Gene Therapy Program and has demonstrated the benefit of such a core facility to the current program project as well as UCSD and other investigators through many venues. The Core laboratory has great expertise in the development and efficient preparation of a variety of high quality research-grade gene transfer vectors, including: adenovirus, multiple serotypes of adeno-associated virus (AAV), lentivirus, retrovirus (ecotropic, amphotropic, and VSV-G pseudotyped), VSV-G virosomes and plasmids. Since its inception in 1995, the Core has accommodated more than 780 requests, many of which have been development/preparation of multiple viral vectors. Technological innovation has also been an important function of the Core laboratory and it has made significant innovations to viral vectors, including improvement in vector production and purification procedures, development of double-strand DNA genome AAV vectors (scAAVs), tetracycline-inducible vectors, tissue-specific and tet-regulated vectors for shRNA/microRNA, and multiple gene expression from a single vector. Core B will work closely with project members, continue technological innovations and quickly incorporate new designs into the service function to provide the most efficient forms of gene transfer vectors to the proposed Program Project.

Public Health Relevance

Every project within the proposed program involves intensive use of viral vectors for gene transfer and will require the expertise of the Core B. Core B will provide to the program participants with the most efficient and innovated form of gene transfer reagents and related services in a centralized manner. The resources are essential to the timely accomplishment of the goal of this program project.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL066941-12
Application #
8743238
Study Section
Heart, Lung, and Blood Program Project Review Committee (HLBP)
Project Start
Project End
Budget Start
2014-07-01
Budget End
2015-06-30
Support Year
12
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Veterans Medical Research Fdn/San Diego
Department
Type
DUNS #
City
San Diego
State
CA
Country
United States
Zip Code
92093
Penny, William F; Hammond, H Kirk (2017) Randomized Clinical Trials of Gene Transfer for Heart Failure with Reduced Ejection Fraction. Hum Gene Ther 28:378-384
Egawa, Junji; Schilling, Jan M; Cui, Weihua et al. (2017) Neuron-specific caveolin-1 overexpression improves motor function and preserves memory in mice subjected to brain trauma. FASEB J 31:3403-3411
Hastings, Randolph H; Montgrain, Philippe R; Quintana, Rick A et al. (2017) Lung carcinoma progression and survival versus amino- and carboxyl-parathyroid hormone-related protein expression. J Cancer Res Clin Oncol 143:1395-1407
Breen, Ellen C; Scadeng, Miriam; Lai, N Chin et al. (2017) Functional magnetic resonance imaging for in vivo quantification of pulmonary hypertension in the Sugen 5416/hypoxia mouse. Exp Physiol 102:347-353
Hammond, H Kirk; Penny, William F; Traverse, Jay H et al. (2016) Intracoronary Gene Transfer of Adenylyl Cyclase 6 in Patients With Heart Failure: A Randomized Clinical Trial. JAMA Cardiol 1:163-71
Gao, Mei Hua; Giamouridis, Dimosthenis; Lai, N Chin et al. (2016) One-time injection of AAV8 encoding urocortin 2 provides long-term resolution of insulin resistance. JCI Insight 1:e88322
Miyanohara, Atsushi; Kamizato, Kota; Juhas, Stefan et al. (2016) Potent spinal parenchymal AAV9-mediated gene delivery by subpial injection in adult rats and pigs. Mol Ther Methods Clin Dev 3:16046
Chen, Chao; Li, Ruixia; Ross, Robert S et al. (2016) Integrins and integrin-related proteins in cardiac fibrosis. J Mol Cell Cardiol 93:162-74
Kassan, Adam; Pham, Uyen; Nguyen, Quynhmy et al. (2016) Caveolin-3 plays a critical role in autophagy after ischemia-reperfusion. Am J Physiol Cell Physiol 311:C854-C865
Cividini, Federico; Scott, Brian T; Dai, Anzhi et al. (2016) O-GlcNAcylation of 8-Oxoguanine DNA Glycosylase (Ogg1) Impairs Oxidative Mitochondrial DNA Lesion Repair in Diabetic Hearts. J Biol Chem 291:26515-26528

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