Core C, The Imaging and Cell Culture Core, incorporates a change in the Core's focus from the current funding period. Currently, Core C serves as the Adenovirus and Cell Culture Core. While it retains those responsibilities, it now incorporates the imaging capabilities that were previously within Core B. Core C will be an integrated resource for comprehensive evaluation of murine phenotypes encompassing gross, microscopic and ultrastructural abnormalities ofthe mouse models prepared and studied by the 3 Projects. In this manner, developing pathology, as visualized at the light and electron microscope levels, can be correlated to overall organ function (Core B). Core C will continue to provide primary cell cultures to the investigators on a weekly basis, if needed, with neonatal rat or mouse fibroblasts and cardiomyocytes being isolated for experiments. The Core will also continue to service the adenovirus needs of the Projects. Imaging needs will be central to analyses ofthe animal models and will incorporate light microscopy, electron microscopy, immunohistochemistry and traditional histopathology. Cell culture needs will continue to be serviced by the Core, as the Projects will require the preparation of both fibroblasts and cardiomyocytes as well. Additionally, during the present funding period, the Core has developed the capabilities of routinely isolating these cells ifrom adult mice, which will be needed by the Projects in this renewal. The Core's Director is an accomplished, very experienced light and electron microscopist, who has focused her entire professional career on the structural analyses of striated muscle. She will be aided by James Gulick, a Research Instructor! who is expert in cell culture, molecular biology and adenoviral preparation and maintenance. All Projects will use this Core.

Public Health Relevance

; Visualization of the changes that occur in the heart tissue and in cardiac cells is very important in understanding the consequences ofthe studies being carried out. This Core will provide those capabilities.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
2P01HL069779-11
Application #
8460279
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2013-09-01
Budget End
2014-05-31
Support Year
11
Fiscal Year
2013
Total Cost
$284,481
Indirect Cost
$92,829
Name
Cincinnati Children's Hospital Medical Center
Department
Type
DUNS #
071284913
City
Cincinnati
State
OH
Country
United States
Zip Code
45229
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Previs, Michael J; Mun, Ji Young; Michalek, Arthur J et al. (2016) Phosphorylation and calcium antagonistically tune myosin-binding protein C's structure and function. Proc Natl Acad Sci U S A 113:3239-44
Gupta, Manish K; McLendon, Patrick M; Gulick, James et al. (2016) UBC9-Mediated Sumoylation Favorably Impacts Cardiac Function in Compromised Hearts. Circ Res 118:1894-905

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