The Bioassay Core will provide biochemical assay and genotyping services for the individual projects within the Program Project. Biochemical assays that will be performed include analyses in plasma and urine for human and rat samples. The following assays will be performed: Plasma renin activity, aldosterone, angiotensin II, cortisol, corticosterone, ACTH, nitrite, nitrate, 8-isoprostane, TEARS, antioxidant capacity, hydrogen peroxide, norepinephrine, epinephrine, albumin, electrolytes, creatinine, total cholesterol, HDL, LDL, triglycerides, glucose, insulin, cystatin C, tetrahydrobiopterin, leptin, and transforming growth factor beta. Techniques will include radioimmunoassay, radioenzymatic assay, HPLC, ELISA, colorimetric, and ionselective electrodes. Genotyping of tagging single nucleotide polymorphisms of the genes of GCCR, CRHR1, p22phox, and EC-SOD will be performed for Project 2. Genotyping of the C-521T polymorphism of the angiotensin II receptor type 1 gene will be performed for Project 1. Genotyping services will be also performed for Project 3 and 4 using the tail snip DMA of the transgenic rats of the ETB receptor deficient rat and endothelial ET knockout mice. The extracted DMA will be genotyped to determine whether the rats are wildtype, heterozygous, or homozygous for the ETB receptor gene and whether the mice are wild-type, heterozygous, or homozygous for the ET gene. The core will also provide resources for development of additional assays as needed by individual investigators. The core will assure assay quality control through validation procedures.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL069999-10
Application #
8376912
Study Section
Special Emphasis Panel (ZHL1-PPG-Z)
Project Start
Project End
2014-02-28
Budget Start
2012-03-01
Budget End
2013-02-28
Support Year
10
Fiscal Year
2012
Total Cost
$434,298
Indirect Cost
$138,857
Name
Georgia Regents University
Department
Type
DUNS #
966668691
City
Augusta
State
GA
Country
United States
Zip Code
30912
De Miguel, Carmen; Speed, Joshua S; Kasztan, Malgorzata et al. (2016) Endothelin-1 and the kidney: new perspectives and recent findings. Curr Opin Nephrol Hypertens 25:35-41
Heimlich, J Brett; Speed, Joshua S; O'Connor, Paul M et al. (2016) Endothelin-1 contributes to the progression of renal injury in sickle cell disease via reactive oxygen species. Br J Pharmacol 173:386-95
Davenport, Anthony P; Hyndman, Kelly A; Dhaun, Neeraj et al. (2016) Endothelin. Pharmacol Rev 68:357-418
Gohar, Eman Y; Giachini, Fernanda R; Pollock, David M et al. (2016) Role of the endothelin system in sexual dimorphism in cardiovascular and renal diseases. Life Sci 159:20-9
Spradley, Frank T; Ho, Dao H; Pollock, Jennifer S (2016) Dahl SS rats demonstrate enhanced aortic perivascular adipose tissue-mediated buffering of vasoconstriction through activation of NOS in the endothelium. Am J Physiol Regul Integr Comp Physiol 310:R286-96
Guan, Zhengrong; Singletary, Sean T; Cha, Haword et al. (2016) Pentosan polysulfate preserves renal microvascular P2X1 receptor reactivity and autoregulatory behavior in DOCA-salt hypertensive rats. Am J Physiol Renal Physiol 310:F456-65
Heimlich, J B; Speed, J S; Bloom, C J et al. (2015) ET-1 increases reactive oxygen species following hypoxia and high-salt diet in the mouse glomerulus. Acta Physiol (Oxf) 213:722-30
Su, Shaoyong; Wang, Xiaoling; Pollock, Jennifer S et al. (2015) Adverse childhood experiences and blood pressure trajectories from childhood to young adulthood: the Georgia stress and Heart study. Circulation 131:1674-81
Speed, Joshua S; Fox, Brandon M; Johnston, Jermaine G et al. (2015) Endothelin and renal ion and water transport. Semin Nephrol 35:137-44
Speed, Joshua S; Pollock, David M (2015) New clues towards solving the mystery of endothelin and blood pressure regulation. Hypertension 66:275-7

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