for the large-scale isolation of coagulation proteins from plasma. Core B personnel will provide the necessary purified proteins for crystallization trials conducted by Dr. James Huntington. Finally, the core will provide some quality control services particularly the quantitation of y-carboxyglutamic acid content to verify the integrity of expressed vitamin K-dependent proteins. The Co-Directors will be responsible for the oversight and efficient conduct of these activities and for ensuring equitable service is provided to the projects.

Public Health Relevance

PROJECT/

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL074124-10
Application #
8450266
Study Section
Special Emphasis Panel (ZHL1-PPG-J)
Project Start
Project End
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
10
Fiscal Year
2013
Total Cost
$230,407
Indirect Cost
$90,342
Name
Children's Hospital of Philadelphia
Department
Type
DUNS #
073757627
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
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Ostertag, Eric M; Kacir, Stephen; Thiboutot, Michelle et al. (2016) ADAMTS13 autoantibodies cloned from patients with acquired thrombotic thrombocytopenic purpura: 1. Structural and functional characterization in vitro. Transfusion 56:1763-74
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Pickens, Brandy; Mao, Yingying; Li, Dengju et al. (2015) Platelet-delivered ADAMTS13 inhibits arterial thrombosis and prevents thrombotic thrombocytopenic purpura in murine models. Blood 125:3326-34
Liang, Hai Po H; Kerschen, Edward J; Basu, Sreemanti et al. (2015) Coagulation factor V mediates inhibition of tissue factor signaling by activated protein C in mice. Blood 126:2415-23

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