The objective of the Metabolism core is to provide the to PPG investigators the scientific and technical support for the execution of biochemical analyses of blood and myocardial tissue samples from all four Projects in the PPG. The Core will perform metabolic and isotopic tracer analysis of blood and tissue from the dogs studied in Projects 1, 2 and 3, and will oversee the experiments with doubly labeled water in mice and dogs in Project 1. The Core will also assess the expression and activity of selected proteins, measure the content of key regulatory metabolites (e.g ATP, lactate, etc), measure mRNA expression for selected genes, and plasma hormone levels. The measurement of Inflamatory cytokines for Project 1 will be performed by the Core. All of the expertise and equipment necessary for these measurements is in place.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
7P01HL074237-10
Application #
8532024
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2013-08-01
Budget End
2014-07-31
Support Year
10
Fiscal Year
2013
Total Cost
$265,459
Indirect Cost
$28,831
Name
Henry Ford Health System
Department
Type
DUNS #
073134603
City
Detroit
State
MI
Country
United States
Zip Code
48202
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