Abstract

A.1. General Goals of the Core The primary purpose of Core C, the genomic and proteomic core (previously Core C) will be to continue providing the principle investigators with cutting edge discovery genomics and proteomics tools to assess and characterize a number of canine and murine pacing models as well as human heart biopsy samples. In essence. Core C will provide crucial experimental services that are integral to the success of each project. It is a centralized facility for the analysis, compilation and correlation of quantitative levels of mRNA, microRNA and a number of key subproteomes and proteins. The proteomics group will also provide detailed analysis of a large number of post-translational modifications (PTM), validation of genomic data using multiple reaction monitoring (MRM), new mass spectrometry (MS) -based quantitative methods along with other MS techniques. In addition, the core has an integrated cellular modeling component that will perform computational modeling to refine and further develop a working cellular model of DHF and CRT.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL077180-10
Application #
8686051
Study Section
Heart, Lung, and Blood Program Project Review Committee (HLBP)
Project Start
Project End
Budget Start
2014-07-01
Budget End
2015-06-30
Support Year
10
Fiscal Year
2014
Total Cost
Indirect Cost

  Institution

Name
Johns Hopkins University
Department
Type
DUNS #
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Wang, Sheng-Bing; Venkatraman, Vidya; Crowgey, Erin L et al. (2018) Protein S-Nitrosylation Controls Glycogen Synthase Kinase 3? Function Independent of Its Phosphorylation State. Circ Res 122:1517-1531
Barth, Andreas S; Kumordzie, Ami; Tomaselli, Gordon F (2016) Orchestrated regulation of energy supply and energy expenditure: Transcriptional coexpression of metabolism, ion homeostasis, and sarcomeric genes in mammalian myocardium. Heart Rhythm 13:1131-1139
Barth, Andreas S; Tomaselli, Gordon F (2016) Gene scanning and heart attack risk. Trends Cardiovasc Med 26:260-5
O'Rourke, Brian; Liu, Ting; Foster, D Brian (2016) Seeing the Forest for the Trees. Circ Res 119:1170-1172
Kwon, Chulan; Tomaselli, Gordon F (2015) Coins of the realm in atrioventricular junction development. Circ Res 116:386-8
Tomaselli, Gordon F (2015) Introduction to a compendium on sudden cardiac death: epidemiology, mechanisms, and management. Circ Res 116:1883-6
Melman, Yonathan F; Shah, Ravi; Danielson, Kirsty et al. (2015) Circulating MicroRNA-30d Is Associated With Response to Cardiac Resynchronization Therapy in Heart Failure and Regulates Cardiomyocyte Apoptosis: A Translational Pilot Study. Circulation 131:2202-2216
Kirk, Jonathan A; Chakir, Khalid; Lee, Kyoung Hwan et al. (2015) Pacemaker-induced transient asynchrony suppresses heart failure progression. Sci Transl Med 7:319ra207
Li, Hui; Lichter, Justin G; Seidel, Thomas et al. (2015) Cardiac Resynchronization Therapy Reduces Subcellular Heterogeneity of Ryanodine Receptors, T-Tubules, and Ca2+ Sparks Produced by Dyssynchronous Heart Failure. Circ Heart Fail 8:1105-14
DeMazumder, Deeptankar; Kass, David A; O'Rourke, Brian et al. (2015) Cardiac resynchronization therapy restores sympathovagal balance in the failing heart by differential remodeling of cholinergic signaling. Circ Res 116:1691-9

Showing the most recent 10 out of 124 publications