Core C is necessary for the following reasons: (i) requirement as outlined in all Projects for centralized imaging with each Project benefitting from high-resolution, state-of-the-art, fluorescent microscopy; and (ii) strong demand for functional studies to quantify microscopic and organ-level endothelial and alveolar epithelial permeability in the murine lung under normal and pathophysiological conditions (e.g., inhaled LPS, cecal ligation and puncture, intratracheal Pseudomonas aeruginosa, and ?double hit? lung injury models). The physiological support component of Core C will provide standardized methods for quantification of lung vascular (Kf,c and Evans blue albumin tracer methods) and alveolar permeability (instilled tracer PS) in wild type and knockout mouse models or after gene delivery via liposomes. The imaging support component of Core C will provide investigators with resources and expertise for live cell and fixed specimen fluorescence, confocal, and two-photon microscopy as well as state-of-the-art facilities for intravital microscopy of the murine lung, important for cell tracking studies proposed in all Projects. Also, Core C will provide assessment of expression of fluorescent or tagged proteins by fluorescence and confocal microscopy. Professor Jahar Bhattacharya, an authority on intravital lung imaging, will serve as consultant to this aspect of the Core function. In summary, the centralized physiological and imaging services offered by Core C will be needed by all Projects in this Program; as such it is indispensable for the overall success of the Program.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL077806-13
Application #
9241425
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Program Officer
Aggarwal, Neil Raj
Project Start
2005-08-01
Project End
Budget Start
2017-04-01
Budget End
2018-03-31
Support Year
13
Fiscal Year
2017
Total Cost
Indirect Cost
Name
University of Illinois at Chicago
Department
Type
DUNS #
098987217
City
Chicago
State
IL
Country
United States
Zip Code
60612
Lv, Yang; Kim, Kyungho; Sheng, Yue et al. (2018) YAP Controls Endothelial Activation and Vascular Inflammation Through TRAF6. Circ Res 123:43-56
Di, Anke; Xiong, Shiqin; Ye, Zhiming et al. (2018) The TWIK2 Potassium Efflux Channel in Macrophages Mediates NLRP3 Inflammasome-Induced Inflammation. Immunity 49:56-65.e4
Dai, Zhiyu; Zhu, Maggie M; Peng, Yi et al. (2018) Endothelial and Smooth Muscle Cell Interaction via FoxM1 Signaling Mediates Vascular Remodeling and Pulmonary Hypertension. Am J Respir Crit Care Med 198:788-802
Mittal, Manish; Nepal, Saroj; Tsukasaki, Yoshikazu et al. (2017) Response by Mittal et al to Letter Regarding Article, ""Neutrophil Activation of Endothelial Cell-Expressed TRPM2 Mediates Transendothelial Neutrophil Migration and Vascular Injury"". Circ Res 121:e87
Soni, Dheeraj; Regmi, Sushil C; Wang, Dong-Mei et al. (2017) Pyk2 phosphorylation of VE-PTP downstream of STIM1-induced Ca2+ entry regulates disassembly of adherens junctions. Am J Physiol Lung Cell Mol Physiol 312:L1003-L1017
Di, Anke; Kiya, Tomohiro; Gong, Haixia et al. (2017) Role of the phagosomal redox-sensitive TRP channel TRPM2 in regulating bactericidal activity of macrophages. J Cell Sci 130:735-744
Reddy, Sekhar P; Mehta, Dolly (2017) Lung Interstitial Macrophages Redefined: It Is Not That Simple Anymore. Am J Respir Cell Mol Biol 57:135-136
Cheng, Kwong Tai; Xiong, Shiqin; Ye, Zhiming et al. (2017) Caspase-11-mediated endothelial pyroptosis underlies endotoxemia-induced lung injury. J Clin Invest 127:4124-4135
Evans, Colin E; Zhao, You-Yang (2017) Molecular Basis of Nitrative Stress in the Pathogenesis of Pulmonary Hypertension. Adv Exp Med Biol 967:33-45
Li, Liping; Sheng, Yue; Li, Wenshu et al. (2017) ?-Catenin Is a Candidate Therapeutic Target for Myeloid Neoplasms with del(5q). Cancer Res 77:4116-4126

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