The Stem Cell and Pathology Core will be a critical resource to this PPG. It will provide the four Projects with access to a wide array of reagents, procedures, and analyses, including i) CPC isolation, sorting, and characterization, ii) assays of CPC function in vitro, and iii) histopathology of cardiac samples obtained in vivo. It will provide the facilities and expertise necessary to isolate, maintain, and manipulate CPCs from several genetically engineered mice. The individual Projects do not have the facilities or expertise needed to isolate and characterize CPCs consistently or to perform the extensive histopathological analyses required. Consolidation of all CPC work into a Core facilitv will decrease the costs of supplies and eguipment because the Core will make bulk purchases of supplies (thereby reducing expenses) and because waste and unnecessary duplication of supplies, reagents, and equipment will be eliminated with the maintenance of centralized stocks and inventories. Consolidating CPC work into a Core facilitv is also time-effective because the techniques involved in this work are very labor intensive and require dedicated, skilled personnel. The Core staff, a full-time team of six dedicated individuals under the immediate supervision of the Core Leaders, will provide consistency and reproducibility of analvsis. This is crucial, because it will ensure that all four Proiects will use CPCs isolated, expanded, and sorted the same way, thereby making the results comparable. A single source of CPCs with rigorous standardization to ensure consistency will result in transplantation of uniform CPC populations in all four Proiects and thus is extremely important for the integration and comparison of results from each of the Projects. Similarly, the uniform histopathologic analyses conducted by the Core in a blinded and rigorously standardized fashion will make it possible to compare results of different experiments within the same Project and among different Projects. The methods used by the Core to prepare CPCs are extremely efficient;CPCs in culture, as determined by c-kit expression (FACS). average 95?3% at passages 2 to 10 and remain at -95% at passage 10. In summary, the Core will ensure guality control and, by eliminating duplication of effort and maximizing the use of personnel and supplies, will enable an efficient use of resources.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Program Projects (P01)
Project #
Application #
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Louisville
United States
Zip Code
Fulghum, Kyle; Hill, Bradford G (2018) Metabolic Mechanisms of Exercise-Induced Cardiac Remodeling. Front Cardiovasc Med 5:127
Hosen, Mohammed Rabiul; Militello, Giuseppe; Weirick, Tyler et al. (2018) Airn Regulates Igf2bp2 Translation in Cardiomyocytes. Circ Res 122:1347-1353
Dassanayaka, Sujith; Zheng, Yuting; Gibb, Andrew A et al. (2018) Cardiac-specific overexpression of aldehyde dehydrogenase 2 exacerbates cardiac remodeling in response to pressure overload. Redox Biol 17:440-449
Osuma, Edie A; Riggs, Daniel W; Gibb, Andrew A et al. (2018) High throughput measurement of metabolism in planarians reveals activation of glycolysis during regeneration. Regeneration (Oxf) 5:78-86
Lindsey, Merry L; Bolli, Roberto; Canty Jr, John M et al. (2018) Guidelines for experimental models of myocardial ischemia and infarction. Am J Physiol Heart Circ Physiol 314:H812-H838
Uchida, Shizuka; Jones, Steven P (2018) RNA Editing: Unexplored Opportunities in the Cardiovascular System. Circ Res 122:399-401
Wysoczynski, Marcin; Khan, Abdur; Bolli, Roberto (2018) New Paradigms in Cell Therapy: Repeated Dosing, Intravenous Delivery, Immunomodulatory Actions, and New Cell Types. Circ Res 123:138-158
Bolli, Roberto; Hare, Joshua (2018) Introduction to a Compendium on Regenerative Cardiology. Circ Res 123:129-131
Gibb, Andrew A; Hill, Bradford G (2018) Metabolic Coordination of Physiological and Pathological Cardiac Remodeling. Circ Res 123:107-128
Hindi, Sajedah M; Sato, Shuichi; Xiong, Guangyan et al. (2018) TAK1 regulates skeletal muscle mass and mitochondrial function. JCI Insight 3:

Showing the most recent 10 out of 193 publications