This Core will provide high-fidelity measures of cardiac physiology for the various mouse lines described in the 3 Projects and provide models of cardiac injury for all Projects to study. Specifically, this Core Unit will be responsible for providing 1) mouse models of cardiac hypertrophy and ischemic injury where both models can lead to heart failure (HF), 2) complete and comprehensive cardiovascular physiology measurements (phenotyping) of all mouse models detailed through the PPG application, and 3) cardiac viral-mediated gene delivery. Surgical mouse models include coronary artery occlusion models for ischemia/reperfusion (l/R) injury or complete coronary artery ligation producing a myocardial infarction (Ml). Also, transverse aortic constriction (TAC) for models of hypertrophy and HF will be handled in this Core. The Core will also help investigators with surgical implantation of AzIet mini-osmotic pumps for chronic drug delivery. Further, cardiac viral-mediated gene delivery can be done either though IV injection or more invasively directly to the heart as seen in this application. Comprehensive mouse cardiovascular physiological phenotyping includes in-depth assessment of murine in vivo cardiac function via echocardiography and hemodynamic measurements via Millar catheterization. Further, this Core will assess conscious blood pressure and electric cardiogram (ECG) using telemetry. This Core will be utilized by all Projects and will continue to be instrumental in providing reproducible and high-quality mouse models of cardiac injury and physiological cardiovascular phenotyping.

Public Health Relevance

Mouse models of cardiac injury have been key tools to delineate novel mechanisms of heart failure development and to also test unique therapeutic strategies for repair of the failing heart. This Core is instrumental to the success of all Projects within this PPG and will continue to provide significant support in animal model and cardiovascular phenotyping.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
2P01HL091799-06A1
Application #
8717110
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2014-05-05
Budget End
2015-04-30
Support Year
6
Fiscal Year
2014
Total Cost
$342,061
Indirect Cost
$122,439
Name
Temple University
Department
Type
DUNS #
057123192
City
Philadelphia
State
PA
Country
United States
Zip Code
19122
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